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Table 3 Identification of different F. philomiragia and F. tularensis subspp. in positive blood culture using FISH.

From: Rapid differentiation of Francisella species and subspecies by fluorescent in situ hybridization targeting the 23S rRNA

 

Bwall1448

(35% FA)

Bwphi1448 + Bwall1448c

(50%FA)

Bwhol1151 + Bwhol1151c

(35%FA)

Bwnov168 + Bwnov168c

(35%FA)

Bwtume168II + Bwtume168c

(20%FA)

Bwmed1379 + Bwmed1379c

(20%FA)

F. tul. subsp. holarctica

+

-

+

-

-

-

F. tul. subsp. mediasiatica

+

-

-

-

+

+

F. tul. subsp. novicida

+

-

-

+

-

-

F. philomiragia

+

+

-

-

-

-

  1. Blood culture bottles were inoculated with 5 ml venous blood spiked with 102CFU of each different strain.
  2. +: positive hybridization
  3. -: negative reaction, no fluorescence