Figure 1

Dot blot hybridizations of identical membranes with EUB 338 (a) and the species-specific probe FIAL (b). PCR-amplified products from F. alocis (field A1) and its closest cultured relative F. villosus (A2) served as positive and negative controls, respectively. Additionally, products from the following bacteria were applied as negative controls: Centipeda periodontii (DSM 2778) (A3), Selenomonas noxia (DSM 19578) (A4), Selenomonas ruminantium (DSM 2150) (A5), Selenomonas lacticifex (DSM 20757) (A6), Selenomonas sputigena (DSM 20758) (A7), Eggerthella lenta (ATCC 25559) (A8), Peptostreptococcus anaerobius (ATCC 27337) (A9), and Actinomyces viscosus (ATCC 15987) (B1), Streptococcus intermedius (ATCC 27335) (B2), Streptococcus mutans (ATCC 35668) (B3), Neisseria lactamica (ATCC 23970) (B4), Flavobacterium odoratum (ATCC 4651) (B5), Fusobacterium necrophorum (NCTC 25286) (B6), Fusobacterium periodonticum (CCUG 14345) (B7), Fusobacterium simiae (CCUG 16798) (B8), F. nucleatum (ATCC 25586) (B9), Klebsiella pneumoniae (ATCC 23357) (C1), Veillonella dispar (ATCC 17748) (C2), Veillonella parvula (ATCC 10790) (C3), Kingella kingae (ATCC 23330) (C4), Eikenella corrodens (CCUG 2138) (C5), Bacteroides fragilis (ATCC 25285) (C6), Bacteroides gracilis (ATCC 33236) (C7), Campylobacter concisus (ATCC 33236) (C8), Campylobacter rectus (ATCC 33238) (C9), Capnocytophaga gingivalis (ATCC 33624) (D1), Capnocytophaga sputigena (ATCC 33612) (D2), Capnocytophaga ochracea (ATCC 27872) (D3), Prevotella buccalis (ATCC 33690) (D4), Prevotella oralis (MCCM 00684) (D5), Prevotella nigrescens (NCTC 9336) (D6), Porphyromonas asaccharolytica (ATCC 25260) (D7), P. intermedia (ATCC 25611) (D8), P. gingivalis (ATCC 33277) (D9), Haemophilus paraphrophilus (ATCC 29241) (E1), Haemophilus aphrophilus (NCTC 55906) (E2), Haemophilus influenzae (clinical isolate) (E3), Haemophilus influenzae (ATCC 33391) (E4), Pasteurella haemolytica (ATCC 33396) (E5), Leptotrichia buccalis (MCCM 00448) (E6), A. actinomycetemcomitans (MCCM 02638) (E7), A. actinomycetemcomitans (ATCC 33384) (E8) and A. actinomycetemcomitans (ATCC 43718) (E9). In columns 10-17 and in lanes F to J of columns 1-9 PCR products from patient samples of the different diseased groups and the periodontitis resistant (PR) group were applied. (a): Signals in all fields prove successful PCR-amplification. (b): Absence of signals in all bacterial controls along with strong signal in field A1 proves specificity of the experiments. Prevalences of F. alocis in all diseased collectives exceed the prevalence in the PR group.