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Figure 5 | BMC Microbiology

Figure 5

From: Modulation of iron homeostasis in macrophages by bacterial intracellular pathogens

Figure 5

Labile iron pool in macrophages during infection with Francisella and Salmonella. RAW264.7 macrophages were infected for 2 h, 8 h, 16 h, and 24 h with wild Francisella (FT), wild-type Salmonella (ST), spiA Salmonella (ST/spiA), or spiC Salmonella (ST/spiC). Labile iron pool was determined with the calcein method as described in detail in Materials and Methods. Measurements were in arbitrary fluorescence units standardized to uninfected samples. Data shown are the deviation in percentage from uninfected samples from triplicate experiments. Results are expressed as means +/- 1 standard error of mean (SEM).

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