Table 3 Primers used in this study.
Primera | Sequence (5'→3') | Purpose |
|---|---|---|
135UpF | CCAAATAACCCGCATATTGAGG | Knockout mleR |
135UpR | GGCGCGCC TTGAAATTTTTCAGCAACCTTA | Knockout mleR |
135DoF | GGCCGGCC TCCTCAACCTTAACACCTGATA | Knockout mleR |
135DoR | GTTGCTAAAGATTTGTTCTCAG | Knockout mleR |
ErmF | GGCGCGCC CCGGGCCCAAAATTTGTTTGAT | ErmEA |
ErmR | GGCCGGCC AGTCGGCAGCGACTCATAGAAT | ErmEA |
lucF | ATATACCATGG AAGACGCCAAAAAC | Luciferase |
lucR | AAAAAAACTAGT TTATGCTAGTTATTGCTCAGCGG | Luciferase |
P135F/EP9 | AAAAAACCATGG CTTTATTCAAAAAAGGATCGTTT | Promoter mleR/EMSA |
P135R | TTTTTTCCATGG TTAACCTTTCTATTATTTTTACTAGTT | Promoter mleR |
P137F/EP6 | AAATTTCCATGG CAAGACTGTTAAAGTCAAAAA | Promoter mleS/EMSA |
P137R/ | AAAAAACCATGG TTTCTGCACCTCCTTATATT | Promoter mleS |
135qF | TGAAGCGTCACCTTGAGAGA | Smu.135 QPCR |
135qR | TAATGGGTGGGCATCCTAAG | Smu.135 QPCR |
136qF | AAGGTATCATCGGCAAGCAC | Smu.136 QPCR |
136qR | TCACTTTTTCAAGCGTCTGC | Smu.136 QPCR |
137qF | GGTATCTTTGCGGCTATGGA | Smu.137 QPCR |
137qR | TTTCACGCAAGACACGAGAG | Smu.137 QPCR |
138qF | CGACGGATAGCAAGTCTGGT | Smu.138 QPCR |
138qR | GTCAACGTGCTAGTCGCAAA | Smu.138 QPCR |
139qF | TACAGCGATTGACGAGAACG | Smu.139 QPCR |
139qR | AGAAATTGGCTTCGCTGAAA | Smu.139 QPCR |
140qF | TTCCTATGCGGATTTTCAGG | Smu.140 QPCR |
140qR | CCTGACCGATTTGGGAATA | Smu.140 QPCR |
1114qF | TACTACCCGGCCCCGATT | Smu.1114 QPCR |
1114qR | CGAGCACGCAAAACAATAGA | Smu.1114 QPCR |
EP1 | TTAACCTTTCTATTATTTTTACTAGTT | EMSA |
EP2 | TCCAAGTGGTTTAAAAGTAACAAGA | EMSA |
EP3 | GCAACTTCCCAAGAGAAAACA | EMSA |
EP4 | TTAATCAAGATTATCAATAATCTC | EMSA |
EP5 | ATGAAGAAAAAAAGCTATCT | EMSA |
EP7 | TGCTTGCCGATGATAGGTT | EMSA |
EP8 | TAAAGAATACAAGTTTAAAAGCAAATAGTTAACT | EMSA |
EP10 | ATAAGTATTTTTTATCCGTTATCTAAGGTTTGAC | EMSA |
EP11 | GTCAAACCTTAGATAACGGATAAAAAATACTTAT | EMSA |