Figure 4
From: Identification and evaluation of the role of the manganese efflux protein in Deinococcus radiodurans
Analysis of the intracellular ion content of wild-type R1 and mntE- cultured in medium supplemented with or without cations. (A) R1 (white bars) and mntE- (grey bars) were cultured in TGY medium supplemented with 50 μM manganese, 10 μM ferric chloride, 100 mM magnesium, or 100 mM calcium chloride to determine the effects of these specific cations. (B) R1 (white bars) and mntE- (grey bars) were cultured in TGY medium without added cations. Cells (OD600 = 0.8) were harvested, and the extracellular cations were removed by washing in EDTA. The cation concentration was determined by ICP-MS. The data represent the means ± standard deviations of three independent experiments.