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Figure 2 | BMC Microbiology

Figure 2

From: Fungal-associated NO is involved in the regulation of oxidative stress during rehydration in lichen symbiosis

Figure 2

ROS in rehydrated R. farinacea thalli. Thalli of R. farinacea rehydrated with deionized water and 10 μM DCFH2-DA and observed 3-4 h post-rehydration. A, B, C, D ROS content, as revealed by the green fluorescence emission of DCF under a fluorescence microscope (magnification: 400× for A, B and 1000× for C, D); E overlay of confocal microscopy images reveals ROS distribution around some of the photobionts (green fluorescence); F overlay of confocal microscopy images of ROS content of R. farinacea thalli that had been rehydrated with c-PTIO 200 μM, arrows point to photobionts photobleached by the confocal laser during the observation (oxPho). Red fluorescence is due to the photobiont's chlorophyll in all cases. Each micrograph is representative of several images corresponding to independent samples. C cortex, M medulla, PL photobiont layer, Pho photobiont, oxPho photobleached photobiont, Hy fungal hyphae

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BMC Microbiology

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