Rhizobium leguminosarum bv. trifolii rosR is required for interaction with clover, biofilm formation and adaptation to the environment

Table 1 rosR mutation affects symbiotic properties and EPS production of R. leguminosarum bv. trifolii 24.2. Defects are fully complemented by the wild-type rosR copy.

Strain/plasmid	Nodule no. per plant^a	Shoot weight (mg/plant)^a		EPS (mg/mg)^b
		(fresh wt)	(dry wt)
Rt2440	5.1 ± 1.9	42.4 ± 11.4	4.3 ± 0.15	0.31 ± 0.03
Rt2441	6.2 ± 2.1	44.8 ± 10.2	4.9 ± 0.20	0.36 ± 0.04
Rt2472	4.9 ± 1.7	43.2 ± 7.7	4.2 ± 0.10	0.30 ± 0.03
Rt2440(pRC24)	12.3 ± 3.1	59.3 ± 12.5	6.1 ± 0.25	1.19 ± 0.07
Rt2441(pRC24)	12.5 ± 3.6	58.8 ± 10.2	6.0 ± 0.2	1.15 ± 0.05
Rt2472(pRC24)	12.7 ± 5.4	61.2 ± 14.2	6.2 ± 0.3	1.21 ± 0.06
Rt24.2 (wild type)	12.8 ± 2.9	62.8 ± 12.1	6.2 ± 0.25	0.97 ± 0.05
Uninoculated clover	-	34.7 ± 6.4	3.8 ± 0.10	-

^a Plants were harvested 28 days after inoculation. Given values ( ± standard deviation) are averages of three independent experiments with 20 plants for each treatment.
^b - Exopolysaccharide (EPS) production (Glc equivalents in mg/mg of protein).

ISSN: 1471-2180