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Table 4 Quantitative RT-PCR analysis on H-NS targets involved in acid stress resistance

From: Decrypting the H-NS-dependent regulatory cascade of acid stress resistance in Escherichia coli

 

Expression ratio

Gene

hns/wild-type

hns gadE /wild-type

hns rcsB /wild-type

hns hdfR /wild-type

hns adiY /wild-type

Glutamate-dependent specific pathway

gadA 1

137.21

nd

Nd

150.93

41.31

dctR 1

34.66

nd

Nd

34.32

8.84

yhiM

10.75

3.41

3.40

10.90

11.36

aslB

12.92

0.66

1.10

0.69

1.32

gltD 1

1.68

nd

Nd

0.48

0.52

Arginine-dependent specific pathway

adiA

16.89

nd

Nd

nd

0.70

adiC

11.62

nd

Nd

nd

1.41

Lysine-dependent specific pathway

cadC

4.62

5.77

6.38

nd

nd

General acid stress resistance pathway

hdeA 1

32.37

nd

Nd

41.20

6.55

hdeD

18.96

nd

Nd

17.57

5.89

adiY

5.08

5.00

5.00

nd

nd

  1. nd: non-determined.
  2. 1: Since several genes are organized in operon and/or are highly homologous to each other, results obtained with gadA also corresponds to gadBC; with gltD to gltB; with hdeA to hdeB; with dctR to slp.
  3. Quantitative RT-PCR were performed on total RNA isolated from exponential growth phase cultures. Standard deviations were less than 20% of the mean.