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Figure 2 | BMC Microbiology

Figure 2

From: Decrypting the H-NS-dependent regulatory cascade of acid stress resistance in Escherichia coli

Figure 2

Competitive gel mobility shift assay with H-NS, target promoter fragments and restriction fragments derived from plasmid pBR322. The cleaved plasmid and promoter fragments were incubated with the indicated concentrations of purified H-NS protein (in μM). After protein-DNA complex formation, the fragments were resolved on a 3% (A) or 4% (B) MetaPhor agarose gel. An asterisk indicates the position of the target promoter fragments. "bla" indicates the bla promoter (positive control), the other fragments of plasmid DNA correspond to negative controls. The specific binding of H-NS is observed when bands corresponding to bla and target promoter disappear with increasing concentration of H-NS, the H-NS-DNA complex being difficult to visualize under these conditions.

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