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Figure 1 | BMC Microbiology

Figure 1

From: Decrypting the H-NS-dependent regulatory cascade of acid stress resistance in Escherichia coli

Figure 1

Gel mobility shift assays with GadE/RcsB D56E complex, HdfR and AdiY. A. Gel mobility shift assays with GadE/RcsBD56E complex and new DNA targets. Proteins were incubated with DNA targets during 30 min at 25°C in the final reaction mixture volume of 15 μl. 900 ng of each GadE and RcsBD56E protein are used for yhiM and aslB. B. Gel mobility shift assays with HdfR or AdiY proteins. Quantities of purified HdfR or AdiY proteins are indicated above each lane (in ng). Gel mobility shift assays (A and B) were performed with 0.1 ng [γ32P]-labelled DNA fragment and loaded on a 6% polyacrylamide native gel. An arrow points out the position of the DNA-regulatory protein complex. An asterisk marks the position of the unbound probe.

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