Differential phenotyping of Brucella species using a newly developed semi-automated metabolic system

BMC Microbiology

Table 2 Brucella strains tested for metabolic activity.

Species	Biovar	Strain	Culture collection	Host	Number of field isolates
					Taxa Profile™ (570 substrates)	Micronaut™ Brucella plate (93 substrates)
	1	544	NCTC^a 10093	Cattle	6	8
	2	86/8/59	NCTC 10501	Cattle	1	1
	3	Tulya	NCTC 10502	Human	4	4
*B. abortus*	4	292	NCTC 10503	Cattle	5	5
	5	B3196	NCTC 10504	Cattle	0	0
	6	870	NCTC 10505	Cattle	3	2
	7*	63175	NCTC 10506	Cattle	0	0
	9	C68	NCTC 10507	Cattle	2	2
	1	16 M	NCTC 10094	Goat	4	7
*B. melitensis*	2	63/9	NCTC 10508	Goat	5	13
	3	Ether	NCTC 10509	Goat	4	10
	1	1330	NCTC 10316	Swine	4	5
	2	Thomsen	NCTC 10510	Swine	6	7
*B. suis*	3	686	NCTC 10511	Swine	1	0
	4	40	AFSSA^b Ref. 40	Reindeer	1	1
	5	513	AFSSA Ref. 513	Wild rodent	0	0
*B. canis*		RM6/66	NCTC 10854	Dog	4	4
*B. ovis*		63/290	NCTC 10512	Sheep	2	3
*B. neotomae*		5K33	NCTC 10084	Desert rat	0	0
*B. pinnipedialis*			NCTC 12890	Common seal	7	7
*B. ceti*			NCTC 12891	Porpoise	0	0
*B. microti*			CCM^c 4915	Common vole	1	9
*B. inopinata*		BO1	BCCN^d 09-01	Human	0	0
*Unknown*			BfR^e 11.1.001/002	Fox	0	2
Total		23 reference strains			60 field isolates	90 field isolates

Brucella reference strains and overview of field isolates tested with the Taxa Profile™ system and the newly developed Brucella specific Micronaut™ microtiter plate.
^a NCTC: National Collection of Type Cultures
^b AFSSA: Agence Française de Sécurité Sanitaire des Aliments
^c CCM: Czech Collection of Microorganisms
^d BCCN: Brucella Culture Collection from Nouzilly
^e BfR: Bundesinstitut für Risikobewertung
* The authenticity of the B. abortus bv 7 reference strain has been questioned; this strain remains as a potential reference strain until an agreement will be finally reached [44].

ISSN: 1471-2180