Iron-sparing Response of Mycobacterium avium subsp. paratuberculosis is strain dependent

BMC Microbiology

Table 3 Transcript and protein expression in cattle MAP under iron-replete (HI) conditions

	MAP ORF ID	Predicted function	^aFold change
			Protein	Transcript
Metabolism
	MAP0150c	FadE25_2 (acyl-coA dehydrogenase)	1.72 ± 0.1	1.88 ± 0.2
	MAP0789	acetyl-CoA acetyltransferase	1.73 ± 0.3	1.56 ± 0.1
	MAP1846c	ATP phosphoribosyltransferase	1.69 ± 0.2	3.68 ± 0.3
	MAP2332c	Fas (fatty acid synthase)	1.61 ± 0.5	2.28 ± 0.4
	MAP3404	AccA3 (acetyl-/propionyl-coenzyme A)	1.45 ± 0.1	2.18 ± 0.2
	MAP3698c	succinate dehydrogenase	1.89 ± 0.3	4.57 ± 0.5
Cellular processes
	MAP1339	iron regulated conserved protein	1.62 ± 0.2	0.78 ± 0.3
	MAP1653	thiol peroxidase	1.79 ± 0.5	2.29 ± 0.2
Information storage and processing
	MAP2907c	translation initiation factor IF-2	1.57 ± 0.2	1.89 ± 0.2
	MAP2945c	ribosome releasing factor	1.66 ± 0.3	2.11 ± 0.5
	MAP4113	50S ribosomal protein L1	1.61 ± 0.1	1.57 ± 0.2
	MAP4125	rplJ 50S ribosomal protein L10	1.52 ± 0.1	1.66 ± 0.5
	MAP4142	fusA elongation factor G	2.13 ± 0.4	3.05 ± 0.3
	MAP4160	rpsJ 30S ribosomal protein S10	1.68 ± 0.3	2.87 ± 0.4
	MAP4181	rpsH 30S ribosomal protein S8	1.79 ± 0.5	2.42 ± 0.1
	MAP4233	rpoA DNA-directed RNA polymerase	1.56 ± 0.1	1.65 ± 0.4
Poorly characterized pathways
	MAP0216	FbpA antigen 85-A	1.87 ± 0.2	2.16 ± 0.3
	MAP1122	mycobacterial integration host factor	1.73 ± 0.3	2.00 ± 0.5

^a MAP oligoarray was used to measure gene expression whereas iTRAQ was used to quantitate protein expression in the cultures of cattle MAP strain grown in iron-replete (HI) or iron-limiting (LI) medium. Fold change for each target was calculated and represented as a log₂ ratio of HI/LI. Shown are the MAP genes that demonstrated the presence of 1.5 times or more of transcripts and proteins in HI compared to LI. Genes are annotated based on the motif searches in KEGG database.

ISSN: 1471-2180