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Figure 3 | BMC Microbiology

Figure 3

From: Iron-sparing Response of Mycobacterium avium subsp. paratuberculosis is strain dependent

Figure 3

Peptide quantitation of proteins expressed by C and S MAP strains under iron-replete conditions: Reporter ion regions (114 - 117 m/z) of peptide tandem mass spectrum from iTRAQ labeled peptides from the (A) 35-kDa major membrane protein (MAP2121c) and (B) BfrA, and the intergenic regions of MAP1508-1509 and MAP2566-2567c. Quantitation of peptides and inferred proteins are made from relative peak areas of reporter ions. Several unique peptides (>95% confidence) were mapped to each protein. However, only one representative peptide is shown for each protein. Peptides obtained from cattle MAP cultures grown in iron-replete and iron-limiting medium were labeled with 114 and 115 reporter ions, respectively. Peptides obtained from sheep MAP cultures grown in iron-replete and iron-limiting medium were labeled with 116 and 117 reporter ions, respectively. The peptide sequences and shown in the parenthesis and the red dashed line illustrates the reporter ion relative peak intensities. MAP2121c alone was upregulated in the sheep MAP strain under iron-replete conditions.

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