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Table 2 NTNH PCR detection in spiked healthy infant stool and a clinical infant botulism sample

From: Universal and specific quantitative detection of botulinum neurotoxin genes

Strain (genomic copies)

NTNH Detection

BoNT A (104)

++

BoNT A (102)

++

BoNT A (10)

+

BoNT A (1)

+

BoNT B (104)

++

BoNT B (102)

+

BoNT B (10)

+

BoNT B (1)

-

BoNT C (104)

++

BoNT C (102)

+

BoNT C (10)

+

BoNT C (1)

-

BoNT D (104)

++

BoNT D (102)

+

BoNT D (10)

-

BoNT D (1)

-

BoNT E (104)

++

BoNT E (102)

+

BoNT E (10)

+

BoNT E (1)

-

BoNT F (104)

++

BoNT F (102)

++

BoNT F (10)

+

BoNT F (1)

-

BoNT G (104)

++

BoNT G (102)

++

BoNT G (10)

+

BoNT G (1)

-

Clinical stool sample

++

  1. ++ Denotes strong PCR band
  2. + Denotes weak PCR band
  3. - Denotes no PCR Band
  4. Listed in this table are results from NTNH gene detection within spiked samples of a healthy infant stool sample as described in the materials and methods. Also included is the result from a confirmed case of infant botulism in California. (++) indicates a strong positive PCR product at the dilution tested, (+) is a weak positive PCR product, and (-) indicates no amplification detected.