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Table 1 Bacterial strains, plasmids and PCR primers used in this study

From: The role of sigma factor RpoH1 in the pH stress response of Sinorhizobium meliloti

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Characteristics

Reference

Sinorhizobium meliloti

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Rm 1021

Spontaneous mutant of wild type strain RU47, Smr

[64]

Rm 1021Ī”rpoE1

Rm1021 derivative, rpoE1 mutant

This study

Rm 1021Ī”rpoE2

Rm1021 derivative, rpoE2 mutant

This study

Rm 1021Ī”rpoE5

Rm1021 derivative, rpoE5 mutant

This study

Rm 1021Ī”rpoH1

Rm1021 derivative, rpoH1 mutant

This study

Rm 1021Ī”fecI

Rm1021 derivative, fecI mutant

This study

Escherichia coli

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DH5_MCR

F- endA1 supE44 thi-1 Ī»- recA1 gyrA96 relA1 deoR Ī”(lacZYA-argF)U169 Ļ•80dlacZ Ī”M15 mcrA Ī”(mrr hsdRMS mcrBC)

[65]

S17-1

E. coli 294::[RP4-2(Tc::Mu)(Km::Tn7)] pro res Ī”recA Tpr

[55]

Plasmids

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pK18mobsacB

pUC18 derivative, sacB lacZ Ī± Kmr, mobilizable

[58]

pJrpoH1

pJN105 derivative, rpoH1, Gmr

This study

Primers

Ā Ā 

DEL_rpoE1_A

AGTAGGATCCGCGATCAGGAGGTCAT

This study

DEL_rpoE1_B

GTCCTTCATCGCTTCGGCAACCGGCATCAATTCCAG

This study

DEL_rpoE1_C

CTGGAATTGATGCCGGTTGCCGAAGCGATGAAGGAC

This study

DEL_rpoE1_D

AGTCGGATCCACGATCCTCTGCGTTGAAGC

This study

DEL_rpoE2_A

ATCGGAATTCGCTCGTCCTCGATGAT

This study

DEL_rpoE2_B

AACGAAGGCACGCGAGGTGACACGCTTGAACTCTTGG

This study

DEL_rpoE2_C

CCAAGAGTTCAAGCGTGTCACCTCGCGTGCCTTCGTT

This study

DEL_rpoE2_D

AGCGGAATTCAACCGCGACGGTTCCTATC

This study

DEL_rpoE5_A

GCGCAAGCTTCTGCAGGATGGAAGCGATT

This study

DEL_rpoE5_B

CTCGTCCGCTCAGTTCAATTGTCGCGATGCGTGACC

This study

DEL_rpoE5_C

GGTCACGCATCGCGACAATTGAACTGAGCGGACGAG

This study

DEL_rpoE5_D

ACGTAAGCTTGCCGACCAGAACCGTAA

This study

DEL_rpoH1_A

CGAAGACAGCGACGATGCAC

This study

DEL_rpoH1_B

ACCAGCCAATCCTGCCACTGCTCGAACTTCTTGACCGCCT

This study

DEL_rpoH1_C

AGGCGGTCAAGAAGTTCGAGCAGTGGCAGGATTGGCTGGT

This study

DEL_rpoH1_D

TATGAAGAGAGGCTCGGCCA

This study

DEL_fecI1_A

CGCGCATTGGTCGTGCGATT

This study

DEL_fecI1_B

GGTGCCGCAGGTACATGTGA

This study

DEL_fecI1_C

TCACATGTACCTGCGGCACCAGGCCTCGACCATGACGAAT

This study

DEL_fecI1_D

GATCGTGCGCCACATCGAAG

This study