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Table 2 Oligonucleotide primers

From: Sialic acid transport and catabolism are cooperatively regulated by SiaR and CRP in nontypeable Haemophilus influenzae

Oligonucleotide primer

Sequence1

Use

140F3

gaa ttc CTG CTT CTT CAT TAA GTT CTC GC

nagA upstream

140R5

ccc ggg CAT ATT CTG TTC CTA ATA TCA ACA TCA GTT

nagA upstream

140F6

ccc ggg TAA TAG TAA ACA CTT AAA TAG TTA ATT GAT TTA AAA ATC

nagA downstream

140R6

gca tgc TCA AAA ACA GCA ACA CGG TGC

nagA downstream

141F1

gaa ttc CAT CAT CGC TGA AAC AGG C

nagB upstream

141R3

ccc ggg CAT ATT AGC CTT CCT TTA TTA TTG ACC G

nagB upstream

141F3

ccc ggg TAG AGA TCT ATT CTT CAT CTT TAT GTA GGG

nagB downstream

141R4

gca tgc GGT TTC AAC GCT AGT TTG GTC G

nagB downstream

144F1

gaa ttc CCG TCC TTT TGT GAA TGT CC

nanK upstream

144F3

ccc ggg CAT AAC TTA TCC TTA TAG TGT AAA GTC TTT TCT CAC

nanK upstream

144F2

ccc ggg AAG CCT GAA GGA ACA ATT TAT GGC TAA

nanK downstream

144R2

gca tgc GCC GTT TCA GCA GAA TAA CCA G

nanK downstream

145F5

gaa ttc CGC TCC TGT GTG AAC TTA TG

nanE upstream

145R5

ccc ggg CAT ATA ACA CCC CTC ATT TAA ATC TGA AT

nanE upstream

145F6

ccc ggg TAG TCG TAA GAC GTG TGA GAA AAG ACT T

nanE downstream

145R6

gca tgc CGA ACG CAA AAT CGT ATC GGC

nanE downstream

604F1

gag ctc CAT TTT GCT GAC GAG GAA CTG

cyaA upstream

604R1

ccc ggg CAT TAC ATA AAC ACC TAA AAT TGG TGG

cyaA upstream

604F2

ccc ggg TAA TAT TTT CCT GTG GTT GAT AGG TTA CC

cyaA downstream

604R2

aag ctt AAA GCA ATG GAG TGG ACC ACA ATT

cyaA downstream

145R8

CCG CAG CAA TTT TTG TCC

PCR SOEing

145M2

TTT ATG AAA AAA CAC TTC AAA AAT

PCR SOEing

145M3

ATT TTT GAA GTG TTT TTT CAT AAA TTA AA T GTG ATC AAC TTC TC

PCR SOEing

146R2

CCA TTA CGG CAC ACT AAA GAG G

PCR SOEing

957F2

aag ctt AAT AAA ACG GAA TTT TTG AAA CAG G

CRP expression

957R2

ctc gag TCT TGC GCC ATA TAC AAC GAT TGT

CRP expression

P146F1

ACA CCC CTC ATT TAA ATC TGA ATA AAT CAC

EMSA

P146R4

CCC CCA AAA TAG GAT TCG

EMSA

145R7

CGA CAG GTT GGC AAG AAG AAA TAA GAC C

Primer extension

145R1

ATC AGC GGC AAG AAC AGC AG

Primer extension

  1. 1Restriction sites added to aid in subcloning are indicated by lowercase letters. Bases added for 5 bp insertion are italicized.