Strain or plasmid | Genotype, relevant phenotype or selection marker | Source or reference |
---|---|---|
Strains | Â | Â |
E. coli DH5α |  | Invitrogen |
E. coli BL21 Star | Â | Invitrogen |
NTHi 2019 | Clinical respiratory isolate | [25] |
JWJ091 | NTHi 2019ΔcyaA mutant | This study |
JWJ093 | NTHi 2019ΔcyaA ΔsiaR mutant, kanamycin resistant | This study |
JWJ112 | NTHi 2019ΔcyaA ΔnanA mutant | This study |
JWJ114 | NTHi 2019ΔcyaA ΔnagA mutant | This study |
JWJ116 | NTHi 2019ΔcyaA ΔnagB mutant | This study |
JWJ118 | NTHi 2019ΔcyaA ΔnanK mutant | This study |
JWJ120 | NTHi 2019ΔcyaA ΔnanE mutant | This study |
JWJ159 | NTHi 2019ΔcyaA mutant with 5 bp insertion between SiaR and Crp operators | This study |
JWJ160 | NTHi 2019ΔcyaA ΔnagB mutant with 5 bp insertion between SiaR and Crp operators | This study |
Plasmids | Â | Â |
pGEM-T Easy | PCR-cloning vector | Promega |
pGEM-T | PCR-cloning vector | Promega |
pCR2.1 | PCR-cloning vector | Invitrogen |
pCR2.1_443 | pCR2.1 with PCR fragment spanning | This study |
pUC19 | General cloning vector | New England Biolabs |
pET-24(+) | Expression vector | Novagen |
pUC19-142del | nanA deletion construct | This study |
pUC19-142sacB | nanA::tetR-sacB/kanR | This study |
pJJ150 | siaR knockout vector | [14] |
pJJ185 | C-terminal his-tagged SiaR expression vector | [14] |
pJJ260 | tetR-sacB/kanRcassette | Â |
pJJ276 | C-terminal his-tagged CRP expression vector | This study |
pJJ279 | cyaA deletion construct | This study |
pJJ290 | cyaA::tetR-sacB/kanR | This study |
pJJ308 | nagA deletion construct | This study |
pJJ309 | nagB deletion construct | This study |
pJJ310 | nanK deletion construct | This study |
pJJ311 | nanE deletion construct | This study |
pJJ313 | nagA::tetR-sacB/kanR | This study |
pJJ314 | nagB::tetR-sacB/kanR | This study |
pJJ315 | nanK::tetR-sacB/kanR | This study |
pJJ316 | nanE::tetR-sacB/kanR | This study |
pJJ321 | pCR2.1_443 with 5 bp deletion | This study |
pJJ331 | pJJ321 with tetR-sacB/kanR | This study |