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Table 1 Strains and plasmids

From: Sialic acid transport and catabolism are cooperatively regulated by SiaR and CRP in nontypeable Haemophilus influenzae

Strain or plasmid

Genotype, relevant phenotype or selection marker

Source or reference

Strains

  

E. coli DH5α

 

Invitrogen

E. coli BL21 Star

 

Invitrogen

NTHi 2019

Clinical respiratory isolate

[25]

JWJ091

NTHi 2019ΔcyaA mutant

This study

JWJ093

NTHi 2019ΔcyaA ΔsiaR mutant, kanamycin resistant

This study

JWJ112

NTHi 2019ΔcyaA ΔnanA mutant

This study

JWJ114

NTHi 2019ΔcyaA ΔnagA mutant

This study

JWJ116

NTHi 2019ΔcyaA ΔnagB mutant

This study

JWJ118

NTHi 2019ΔcyaA ΔnanK mutant

This study

JWJ120

NTHi 2019ΔcyaA ΔnanE mutant

This study

JWJ159

NTHi 2019ΔcyaA mutant with 5 bp insertion between SiaR and Crp operators

This study

JWJ160

NTHi 2019ΔcyaA ΔnagB mutant with 5 bp insertion between SiaR and Crp operators

This study

Plasmids

  

pGEM-T Easy

PCR-cloning vector

Promega

pGEM-T

PCR-cloning vector

Promega

pCR2.1

PCR-cloning vector

Invitrogen

pCR2.1_443

pCR2.1 with PCR fragment spanning

This study

pUC19

General cloning vector

New England Biolabs

pET-24(+)

Expression vector

Novagen

pUC19-142del

nanA deletion construct

This study

pUC19-142sacB

nanA::tetR-sacB/kanR

This study

pJJ150

siaR knockout vector

[14]

pJJ185

C-terminal his-tagged SiaR expression vector

[14]

pJJ260

tetR-sacB/kanRcassette

 

pJJ276

C-terminal his-tagged CRP expression vector

This study

pJJ279

cyaA deletion construct

This study

pJJ290

cyaA::tetR-sacB/kanR

This study

pJJ308

nagA deletion construct

This study

pJJ309

nagB deletion construct

This study

pJJ310

nanK deletion construct

This study

pJJ311

nanE deletion construct

This study

pJJ313

nagA::tetR-sacB/kanR

This study

pJJ314

nagB::tetR-sacB/kanR

This study

pJJ315

nanK::tetR-sacB/kanR

This study

pJJ316

nanE::tetR-sacB/kanR

This study

pJJ321

pCR2.1_443 with 5 bp deletion

This study

pJJ331

pJJ321 with tetR-sacB/kanR

This study