Figure 2

Primer extension analysis of the nan and siaPT operons. Complement of sequence surrounding the transcriptional start sites included with the sites indicated in bold and with asterisks. A. Primer extension analysis identified at least two major transcriptional start sites for the nan operon. Two bands were present for TS-2 _nan as indicated. B. Primer extension identified one start site for the siaPT operon. C. Schematic diagram of the nan and siaPT promoters. Binding sites for SiaR (red box) and CRP (blue box) are indicated as well as putative -10 boxes for TS-1 _nan and TS-1 _siaPT (yellow boxes).