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Table 6 Strains and plasmids used in this study

From: Identification of the full set of Listeria monocytogenes penicillin-binding proteins and characterization of PBPD2 (Lmo2812)

Strain or plasmid

Relevant genotype and features

Reference or source

strains

EGD

L. monocytogenes wild-type

 

KD2812

Δlmo2812 derivative of EGD

This work

AD07

Δlmo2754 derivative of KD2812

This work

E. coli DH5α

F- Φ80 Δ lac ZM15(lac ZYA-org F) U169 deo R rec A1 end A1 hsd R17 pho A sup E44kλ-thi-1 gyr A96 rel A1

 

E. coli BL21(DE3)

F- omp T gal dcm hsd SB(rB- mB-) λ(DE3)

Novagen

plasmids

pET30a

 

Novagen

pAD3

pET30a derivative containing lmo2812 gene

This work

pKSV7

temperature-sensitive integration vector; MCSa; lac Z; β-lac; cat, pE194 Ts rep

[31]

pKD2812

pKSV7 carrying the Δlmo2812 allele

This work

pADPBP5

pKSV7 carrying the Δlmo2754 allele

This work

  1. aMCS - multiple cloning site