Strain or plasmid | Relevant genotype and features | Reference or source |
---|---|---|
strains | ||
EGD | L. monocytogenes wild-type | |
KD2812 | Δlmo2812 derivative of EGD | This work |
AD07 | Δlmo2754 derivative of KD2812 | This work |
E. coli DH5α | F- Φ80 Δ lac ZM15(lac ZYA-org F) U169 deo R rec A1 end A1 hsd R17 pho A sup E44kλ-thi-1 gyr A96 rel A1 | |
E. coli BL21(DE3) | F- omp T gal dcm hsd SB(rB- mB-) λ(DE3) | Novagen |
plasmids | ||
pET30a | Novagen | |
pAD3 | pET30a derivative containing lmo2812 gene | This work |
pKSV7 | temperature-sensitive integration vector; MCSa; lac Z; β-lac; cat, pE194 Ts rep | [31] |
pKD2812 | pKSV7 carrying the Δlmo2812 allele | This work |
pADPBP5 | pKSV7 carrying the Δlmo2754 allele | This work |