Figure 7
Mycobacteria do not induce rapid apoptosis in BMDM originating from C57Bl/6 mice. A. Differentiated C57Bl/6 BMDMs were infected at an MOI of 10:1 with M. smegmatis (Msme), M. fortuitum (Mfort), M. kansasii (Mkan), M. bovis BCG or left untreated (UT). The percentage of apoptotic cells was determined using a propidium iodide based staining protocol to detect the population of hypodiploid cells via flow cytometry at 20 h after infection. B. C57Bl/6 BMDMs were infected as in A. or incubated with staurosporine (ST) and the amount of apoptosis was detected using TUNEL staining and flow cytometry analysis. C. Macrophages were infected at MOIs of 1:1, 3:1, and 10:1 with M. smegmatis (Msme), M. fortuitum (Mfort), M. kansasii (Mkan), M. bovis BCG, or left untreated (UT). Culture supernatants of triplicate wells were collected after 20 h and the amounts of secreted TNF was determined using ELISA. In A. and B. the data shown is the mean and standard deviation of three independent experiments. In C. the values are the mean and standard deviation of triplicate readings of one experiment and they are representative of three independent experiments.