Figure 3

Phylogenetic tree of autotransporter adhesins including AatA. The phylogenetic trees were calculated with the Neighbor-Joining-Algorithm on the basis of a ClustalW multiple alignment of 24 protein sequences from known adhesins of the autotransporter family including AatA. The percentages of replicate trees in which the associated taxa clustered together in the bootstrap test (1000 replicates) are shown next to the branches. Protein sequences were obtained from the NCBI database. A: Phylogenetic tree (NJ-tree) obtained using the complete 24 protein sequences. B: NJ-tree obtained using only the last 256 amino acid residues according to the smallest protein HadA in ClustalW analyses. Here, only proteins clustering in one phylogenetic branch with AatA are shown. C: The amino acid residue alignment of the C-termini of AIDA-I and AatA are shown highlighting identical residues (*indicates fully conserved residues, :indicates fully conserved strong groups, .indicates fully conserved weaker groups). Symbols indicate the species: *Escherichia coli, ^#Neisseria meningitidis, °Haemophilus influenzae, ⁺Yersinia enterocolitica, 'Moraxella catarrhalis, ´´Helicobacter pylori, ^$Xylella fastidiosa, **Salmonella Typhimurium, and ^&Bordetella pertussis.