Genes and pathways for CO2 fixation in the obligate, chemolithoautotrophic acidophile, Acidithiobacillus ferrooxidans, Carbon fixation in A. ferrooxidans

Table 1 List of bacterial strains and plasmids used in this study

Strain or plasmid	Relevant characteristic	Source or reference
Bacterial strains
Acidithiobacillus ferrooxidans	Type strain	ATCC 23270
E. coli TOP10	F^- mcr A Δ(mrr-hsd RMS-mcr BC) ϕ80lac ZΔM15 Δlac X74 rec A1 ara D139 Δ(ara-leu) 7697 gal U gal K rps L (Str^R) end A1 nup G	Invitrogen
Plasmids
pBAD-TOPO^®	Amp^R promoter ara BAD (P_BAD) C-terminal: V5 epitope tag-polyhistidine (6 × His)	Invitrogen
pBAD-cbb R	pBAD-TOPO::927-bp fragment containing cbb R from A. ferrooxidans ATCC 23270 expressed from P_BAD promoter	This study

Abbreviations used: ATCC, American Type Culture Collection. Amp^R, ampicillin resistance; Str^R, streptomycin resistance.

ISSN: 1471-2180