Figure 4

TIP60 is required for efficient Salmonella replication. Transfected macrophages were infected with wild-type S. typhimurium or the sseF mutant strains at an MOI of 10. Extracellular bacteria were removed by washing and gentamicin treatment. At 2 and 24 h after bacterial invasion, cells were lysed, and the number of intracellular bacteria was enumerated. The data shown were obtained from three independent experiments with standard errors. The effect of TIP60 knockdown is verified by Western blot using the anti-TIP60 antibodies. Actin was used a control.