Figure 6
From: Characterization and genomic analysis of chromate resistant and reducing Bacillus cereus strain SJ1
RT-PCR analysis of chrA, chrI induction and chrI-chrA 1 co-transcription. The M, r, c, g were identical to these of Figure 5. (A), RT-PCR analysis of expression of chrA's. Lanes 1-7, chromate resistance gene chrA 1 (locus_tag: BCSJ1_04594, 946 bp); Lanes 8-14, chrA 2 (locus_tag: BCSJ1_18833, 491 bp); Lanes 15-21, chrA 3 (locus_tag: BCSJ1_18828, 354 bp). (B), RT-PCR analysis of chrI induction and chrI-chrA 1 co-transcription. 5 and 15 after r and c represent samples induced by 0.3 mM K2CrO4 for 5 min and 15 min, respectively. Lanes 1-7, transcriptional regulator gene chrI (locus_tag: BCSJ1_04599, 604 bp); Lanes 8-14, chrI-chrA 1 (1,130 bp). Lanes 15-17, RT-PCR of 16 S rRNA genes. The arrow indicates a non-specific band.