Figure 5
From: Characterization and genomic analysis of chromate resistant and reducing Bacillus cereus strain SJ1
RT-PCR analysis of putative chromate reduction genes nitR and azoR. M, 1 kb DNA ladder. r, negative control for RT, obtained using total RNA (after DNase I treatment) as the template for PCR amplification, to verify that no genomic contamination was present in the RNA extract; c, RT-PCR product using the first strand cDNA as the template; g, PCR positive control obtained using genomic DNA from B. cereus SJ1 as the template. 0, 1 and 3 after r and c represent samples uninduced and induced by 0.3 mM K2CrO4 for 1 h and 3 h, respectively. Lanes 1-7, nitR 1 (locus_tag: BCSJ1_00500, 592 bp); Lanes 8-14, azoR (locus_tag: BCSJ1_06081, 413 bp); Lanes 15-21, nitR 2 (locus_tag: BCSJ1_14230, 480 bp); Lanes 22-28, nitR 3 (locus_tag: BCSJ1_17540, 546 bp); Lanes 29-35, nitR 4 (locus_tag: BCSJ1_02410, 477 bp); Lanes 36-38, RT-PCR of 16 S rRNA genes. The arrow indicates a non-specific band.