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Table 1 Bacterial strains and plasmids used in the study.

From: Characterization and functional analysis of seven flagellin genes in Rhizobium leguminosarum bv. viciae. Characterization of R. leguminosarum flagellins

Strains and Plasmids

Relevant characteristics

Source or Reference

Escherichia coli strains

  

DH5α

endA1, hsdR17, supE44, thi-1, recA1, gyrA96, relA1,(argF-lacZYA), U169, φ 80dlacZ ΔM15

Invitrogen

S17.1

Spr. RP4 tra region, mobilizer strain

[69]

Rhizobium leguminosarum strains

  

3841

biovar viciae, JB300 derivative, Smr

[70]

VF39SM

biovar viciae, Smr

[71]

VF39SM flaA-

VF39SM flaA-, Smr,Nmr

This work

VF39SMflaA+

VF39SMflaA- complemented with flaA, Smr, Nmr,Gmr

This work

3841 flaA-

gusA-Nm cassette insertion in 3841 flaA, Smr, Nmr

This work

3841flaA+

3841flaA- complemented with flaA, Smr, Nmr,Gmr

This work

VF39SM flaB-

Spectinomycin cassette insertion in VF39SM flaB, Smr,Spr

This work

3841 flaB-

Spectinomycin cassette insertion in 3841 flaB, Smr, Spr

This work

VF39SM flaC-

gusA-Nm cassette insertion in VF39SM flaC, Smr, Nmr

This work

3841 flaC-

gusA-Nm cassette insertion in 3841 flaC, Smr, Nmr

This work

VF39SM flaD-

gusA-Nm cassette insertion in VF39SM flaD, Smr, Nmr

This work

3841 flaD-

gusA-Nm cassette insertion in 3841 flaD, Smr, Nmr

This work

VF39SM flaE-

gusA-Nm cassette insertion in VF39SM flaE, Smr, Nmr

This work

3841 flaE-

gusA-Nm cassette insertion in 3841 flaE, Smr, Nmr

This work

VF39SM flaH-

Neomycin-resistance cassette insertion in VF39SM flaH, Smr, Nmr

This work

3841 flaH-

Neomycin-resistance cassette insertion in 3841 flaH, Smr, Nmr

This work

VF39SM flaG-

Tetracycline-resistance cassette insertion in VF39SM flaG, Smr, Tcr

This work

3841 flaG-

Tetracycline-resistance cassette insertion in 3841 flaG, Smr, Tcr

This work

3841 flaA/B/C/D-

3841 strain with mutations in flaA/B/C/D, Smr, Nmr

This work

VF39SM flaA/B/C/D-

VF39SM strain with mutations in flaB/C/D, Smr, Nmr

This work

VF39SM flaB/C/D-

VF39SM flaA/B/C/D - complemented with flaA; Smr, Nmr, Gmr

This work

3841 flaB/C/D -

3841 flaA/B/C/D - complemented with flaA; Smr, Nmr , Gmr

This work

Plasmids

  

pCR2.1-TOPO

Cloning vector, Ampr , Kmr

Invitrogen

pJQ200SK

Suicide vector with sacB system; Gmr

[32]

pJQ200mp18

Suicide vector with sacB system; Gmr

[32]

pCRS530

Contains a promoterless gusA-Nm cassette

[33]

pBSL99

Contains kanamycin-resistance cassette

[36]

pBSIISK+

Cloning vector, Ampr

Stratagene

pBS::flaD 3'-Km-flaA 5'

flaA 5' fragment (from pCR2.1::flaA 5') subcloned into pBS::flaD 3'-Km, Ampr , Kmr

This work

pJQmp18:: flaD 3'-Km-flaA 5'

flaD 3'-Km-flaA 5' fragment subcloned from pBS::flaD 3'-Km-flaA 5' into pJQmp18, Gmr , Kmr

This work

pHP45Ω

Contains omega-spectinomycin cassette; Spr

[34]

pHP45:ΩTc

Contains tetracycline-resistance cassette; Tcr

[35]

pBBR1-MCS5

Broad-host-range cloning vector, Gmr

[72]

pFus1

pMP220 derivative with promoterless gusA, Tcr

[33]

pBBR1-MCS5:3841flaA

Broad-host-range cloning vector containing flaA gene (with promoter region) from 3841

This work

pBBR1-MCS5:VF39SMflaA

Broad-host-range cloning vector containing flaA gene (with promoter region) from VF39SM

This work

pFus1::flaB

flaB promoter introduced into pFus1, Tcr

This work

  1. AprAmpicillin resistance, GmrGentamicin resistance, NmrNeomycin resistance, SmrStreptomycin resistance, SprSpectinomycin resistance, TcrTetracycline resistance