Strains and Plasmids | Relevant characteristics | Source or Reference |
---|---|---|
Escherichia coli strains | Â | Â |
DH5α | endA1, hsdR17, supE44, thi-1, recA1, gyrA96, relA1,(argF-lacZYA), U169, φ 80dlacZ ΔM15 | Invitrogen |
S17.1 | Spr. RP4 tra region, mobilizer strain | [69] |
Rhizobium leguminosarum strains | Â | Â |
3841 | biovar viciae, JB300 derivative, Smr | [70] |
VF39SM | biovar viciae, Smr | [71] |
VF39SM flaA- | VF39SM flaA-, Smr,Nmr | This work |
VF39SMflaA+ | VF39SMflaA- complemented with flaA, Smr, Nmr,Gmr | This work |
3841 flaA- | gusA-Nm cassette insertion in 3841 flaA, Smr, Nmr | This work |
3841flaA+ | 3841flaA- complemented with flaA, Smr, Nmr,Gmr | This work |
VF39SM flaB- | Spectinomycin cassette insertion in VF39SM flaB, Smr,Spr | This work |
3841 flaB- | Spectinomycin cassette insertion in 3841 flaB, Smr, Spr | This work |
VF39SM flaC- | gusA-Nm cassette insertion in VF39SM flaC, Smr, Nmr | This work |
3841 flaC- | gusA-Nm cassette insertion in 3841 flaC, Smr, Nmr | This work |
VF39SM flaD- | gusA-Nm cassette insertion in VF39SM flaD, Smr, Nmr | This work |
3841 flaD- | gusA-Nm cassette insertion in 3841 flaD, Smr, Nmr | This work |
VF39SM flaE- | gusA-Nm cassette insertion in VF39SM flaE, Smr, Nmr | This work |
3841 flaE- | gusA-Nm cassette insertion in 3841 flaE, Smr, Nmr | This work |
VF39SM flaH- | Neomycin-resistance cassette insertion in VF39SM flaH, Smr, Nmr | This work |
3841 flaH- | Neomycin-resistance cassette insertion in 3841 flaH, Smr, Nmr | This work |
VF39SM flaG- | Tetracycline-resistance cassette insertion in VF39SM flaG, Smr, Tcr | This work |
3841 flaG- | Tetracycline-resistance cassette insertion in 3841 flaG, Smr, Tcr | This work |
3841 flaA/B/C/D- | 3841 strain with mutations in flaA/B/C/D, Smr, Nmr | This work |
VF39SM flaA/B/C/D- | VF39SM strain with mutations in flaB/C/D, Smr, Nmr | This work |
VF39SM flaB/C/D- | VF39SM flaA/B/C/D - complemented with flaA; Smr, Nmr, Gmr | This work |
3841 flaB/C/D - | 3841 flaA/B/C/D - complemented with flaA; Smr, Nmr , Gmr | This work |
Plasmids | Â | Â |
pCR2.1-TOPO | Cloning vector, Ampr , Kmr | Invitrogen |
pJQ200SK | Suicide vector with sacB system; Gmr | [32] |
pJQ200mp18 | Suicide vector with sacB system; Gmr | [32] |
pCRS530 | Contains a promoterless gusA-Nm cassette | [33] |
pBSL99 | Contains kanamycin-resistance cassette | [36] |
pBSIISK+ | Cloning vector, Ampr | Stratagene |
pBS::flaD 3'-Km-flaA 5' | flaA 5' fragment (from pCR2.1::flaA 5') subcloned into pBS::flaD 3'-Km, Ampr , Kmr | This work |
pJQmp18:: flaD 3'-Km-flaA 5' | flaD 3'-Km-flaA 5' fragment subcloned from pBS::flaD 3'-Km-flaA 5' into pJQmp18, Gmr , Kmr | This work |
pHP45Ω | Contains omega-spectinomycin cassette; Spr | [34] |
pHP45:ΩTc | Contains tetracycline-resistance cassette; Tcr | [35] |
pBBR1-MCS5 | Broad-host-range cloning vector, Gmr | [72] |
pFus1 | pMP220 derivative with promoterless gusA, Tcr | [33] |
pBBR1-MCS5:3841flaA | Broad-host-range cloning vector containing flaA gene (with promoter region) from 3841 | This work |
pBBR1-MCS5:VF39SMflaA | Broad-host-range cloning vector containing flaA gene (with promoter region) from VF39SM | This work |
pFus1::flaB | flaB promoter introduced into pFus1, Tcr | This work |