Table 3 Bacterial strains and plasmids

E. coli DH5α

Sup E 44 ΔlacU169(Φ80 lacZ ΔM15) hsd R 17 recA 1 endA 1gyrA 96 thi-1 rel A 1

Gibco-BRL

P. aeruginosa PAO1

Wild type prototroph

Stephen Lory's Lab

P. aeruginosa PCA

PAO1 ΔphzH ΔphzS ΔphzM

This work

Plasmid pDN18

RK2-derived cloning vector, Tet^R

Stephen Lory's Lab, [18]

pBluescript II KS (+)

Universal cloning vector, Amp^R

Stratagene

pEX18Ap

Gene replacement vector, oriT⁺ sacB⁺, Amp^R

Stephen Lory's Lab, [16]

pBAD18

Vector containing araC gene and P _BAD promoter, Amp^R

[35]

pRKaraRed

Broad-host-range, lambda Red proteins expression vector, Tet^R

This work