Table 1 Efficiencies of pRKaraRed-mediated recombination under different conditions
Conditions | Positive colonies/Growing colonies (%)a | Overall efficiency (%) | |
|---|---|---|---|
Replacement with marker genesb | Deletion of marker genesc | ||
A. L-arabinose concentration | |||
0.05% | 10/19 (53%) | 9/20 (45%) | 24% |
0.1% | 31/43 (72%) | 17/20 (85%) | 61% |
0.2% | 67/68 (99%) | 20/20 (100%) | 99% |
0.4% | 62/63 (98%) | 20/20 (100%) | 98% |
0.8% | 70/73 (96%) | 20/20 (100%) | 96% |
1.0% | 59/61 (97%) | 19/20 (95%) | 92% |
B. Length of homology regions | |||
50 bp | 66/67 (99%) | 20/20 (100%) | 99% |
60 bp | 72/73 (99%) | 20/20 (100%) | 99% |
100 bp | 79/80 (99%) | 20/20 (100%) | 99% |
C. Induction time | |||
1 hours | 33/39 (85%) | 17/20 (85%) | 72% |
3 hours | 63/64 (98%) | 20/20 (100%) | 98% |
6 hours | 56/57 (98%) | 20/20 (100%) | 98% |
12 hours | 48/49 (98%) | 19/20 (95%) | 93% |