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Figure 1 | BMC Microbiology

Figure 1

From: Scarless and sequential gene modification in Pseudomonas using PCR product flanked by short homology regions

Figure 1

Map of plasmid pRKaraRed. Some restriction sites are shown. tet A is the tetracycline resistance gene for plasmid selection in E. coli and in P. aeruginosa. oriT is a region for plasmid transfer in P. aeruginosa. Expression of lambda Red genes (gam, bet and exo) driven by P BAD promoter are regulated by repressor AraC. The nucleotide sequence of pRKaraRed was deposited in GenBank under the accession number GU186864.

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