Figure 4

BN-PAGE separation of the T. brucei gambiense secretome (OK strain). Proteins were separated by native gel electrophoresis (BN-PAGE) and stained with coomassie brilliant blue. Coomassie-stained protein spots (186) were excised, digested with trypsin, and identified by MS/MS. 382 proteins were identified and the associated data (accession numbers, molecular masses and MS/MS data) are presented in additional file 2, Table S2.