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Table 1 Adhesion of C. diphtheriae to epithelial cells and internalization. D562 cells (2 × 105 cells per well) were infected with C. diphtheriae (4 × 107 cfu/ml) leading to a multiplicity of infection (MOI) of 200.

From: Corynebacterium diphtheriae invasion-associated protein (DIP1281) is involved in cell surface organization, adhesion and internalization in epithelial cells

Strain

Viable bacteria (CFU/ml)a

 

adherentb

internalizedc

ISS3319

10.1 × 105 ± 1.4 × 105

1.6 × 103 ± 1.0 × 102

ISS4060

3.5 × 105 ± 1.0 × 105

3.0 × 103 ± 1.4 × 103

Lilo1

1.6 × 102 ± 2.1 × 102

n. d.

Lilo2

9.3 ± 10.6

n. d.

  1. a values represent the means and standard deviations of three separate experiments
  2. b average number of bacteria recovered on agar plates after 1.5 h of infection
  3. c average number of bacteria recovered on agar plates after 1.5 h of infection and further 2 h of treatment with gentamicin
  4. n. d.: not detectable
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BMC Microbiology

ISSN: 1471-2180

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