Figure 6

Confocal laser scanning microscope images of S. mutans biofilms in the absence (A) or presence (B) of 0.5 μM carolacton after 12 h of anaerobic cultivation. Staining using the LIVE/DEAD BacLight Bacterial Viability Kit assessed bacterial viability: green areas indicate live cells; red areas indicate dead or damaged cells. The top panel shows a bird's eye view on the biofilm with lines indicating the position of the vertical sections shown at the lower and right margins of both images. Acquired using an UPLSAPO 20× objective lens, size of scale bar 50 μm. The bottom panel shows enlarged horizontal sections of S. mutans biofilms in the absence (A) or presence (B) of 0.5 μM carolacton, aacquired using an UPLSAPO 40× objective lens with 7× digital magnification, size of scale bar 5 μm.