Table 1 Primers used for preparation of probes

GTTGCGGACGTGTGACTTG

to detect the left extremity of chromosome

GAACTACATGCCGGGAGTG

CAGCAAGGATACGGGGAC

to detect the avermectin biosynthetic gene cluster

ACCGAGCACGATGCCGATG

GGTTGACGACGTCCTTGAG

to detect fragment Ase I-G1

CGACACTCATGAAGCGACC

CTGCTCAAGACGAAGGTGC

to detect fragment Ase I-G2

CCGTCACATCGCTGTCATG

GTGATCGTGAAGACCTCGC

to detect fragment Ase I-D

CTCCACCATGACAAGACCG

AGTCGTACGTCCGCAACTG

to detect the right extremity of chromosome

AGGTCTTCCGCTTCGCTTC

CTCTAGACGGCGGAATCAC

to detect the duplication and translocation of right 88-kb end

GCGACAAGGGCTAAGACTC

GTTCTGGCAGTCGTCGTAG

to detect the duplication and translocation of right 88-kb end

TGAAGAAGACCCGGTCTGG

ACGAACGTGCCCTGCTCAC

to detect the duplication and translocation of right 88-kb end

GGTGACGAGTTCGGAGACG

GGAGGTAGCGGATGTTGTG

to detect the rearrangement event in fragment Ase I-NA1

CTGGTCCCAGTACACGATC

GGCTCGTTCATCTTCCTCG

to detect the rearrangement event in fragment Ase I-NA2

GCACATCAGAGGGTCATGC

CGTTGACGTAGAGCTGCG

to detect the rearrangement event in fragment Ase I-NA3

ACCTGAGCAGCTCGTGAAG

TGTGACGGTGTGCCAGTAG

to detect the rearrangement event in fragment Ase I-NA4

ATGCCCTCGACTACGACAC