Skip to main content
Figure 3 | BMC Microbiology

Figure 3

From: Inorganic pyrophosphatase in uncultivable hemotrophic mycoplasmas: identification and properties of the enzyme from Mycoplasma suis

Figure 3

Expression and immunological characterization of the M. suis sPPase. (A) Coomassie-stained SDS polyacrylamide gel electrophoresis of recombinant M. suis PPase., Co, non-induced IMAC purified E. coli lysate; PPA, IMAC purified recombinant PPase. (B) Immunoblot analysis of recombinant PPase and M. suis whole cell antigen; immunological detection with anti-PPase rabbit immune serum; M, molecular weight standard; PPA, recombinant PPase; Ms, purified M. suis cells; Co, non-induced IMAC purified E. coli lysate. (C) Immunoblot of recombinant PPAse; immunological detection with a serum pool from experimentally infected pigs; PPA, recombinant PPase; Co, non-induced IMAC purified E. coli lysate.

Back to article page

BMC Microbiology

ISSN: 1471-2180

Contact us