Table 2 Specific PCRs for identification of plasmid and chromosomally inherited α-hly determinants
From: Common origin of plasmid encoded alpha-hemolysin genes in Escherichia coli
DNA-target (position in sequence) | GenBank Accession | Primer | nucleotide sequence (5' - 3') | Tm (°C) | PCR product bp |
|---|---|---|---|---|---|
hlyA (1915-1936) (2560-2580) | FM180012 | 10f 10r | GCTGCAAATAAATTGCACTCAG CCCTGCACCGATATTATCAAG | 53.1 | 666 |
"pHly152" (953-974) &hlyC (1612-1630) | FM180012 | 1f 1r | GTAGTTCAAAAGACAACTCGTG ATCCCCGAAAGGAGCAATC | 50.6 | 678 |
hlyR (597-618) & "pHly152" (1246-1267) | FM180012 | 32f 32r | GTCTTGCCGTACAATAATTTCC TCCGTTTAATGTCATAACTCGC | 56.5 | 671a |
hlyR (167-188) (830-851) | FM180012 | 44f 44 | ATTCCAAGCGAAGTCCATCCCC CATAAAGCATGATGCCACCACG | 66.5 | 685a |
hlyA (3817-3839) (4497-4518) | FM180012 | 111f 111r | GATGGCACAAAAGCAACCGAAG TTCTCGCTTGAAGGCCACATCC | 55.4 | 702 |
hlyA (3865-3883) (4592-4613) | FM180012 | 113f 113r | CTTGGTGGCGATGTTAAGG GACTCTTTTTCAAACCAGTTCC | 53.5 | 749 |
hlyD (8297-8319) & IS911 (8925-8946) | FM180012 | 99f 99r | GCAGAATGCCATCATTAAAGTG CCATGTAGCTCAAGTATCTGAC | 53.8 | 650 |
PAI I (536) (44506-44524) &hlyC (45278-45299) | AJ488511 | 81f 81r | CCTGTGACACTTCTCTTGC CCCAAGAACCTCTAATGGATTG | 52.3 | 773a |
PAI II (536) (31974-31995) &hlyC (32650-32668) | AJ494981 | 72f 72r | CCCAACTACAATATGCAACAGG CGCCAATAGAGTTGCCTTC | 51.9 | 695 |