Table 3 Melting temperature (Tm) of each primer according to the number of mismatches allowed between the primer and the target sequence.
From: ITS as an environmental DNA barcode for fungi: an in silico approach reveals potential PCR biases
Primer | Number of mismatches allowed | |||
|---|---|---|---|---|
0 | 1* | 2* | 3* | |
ITS1(1) ** | 58.64 | 51.75+/-2.88 | 46.51+/-0.6 | 41.4+/-NA |
ITS1(2) ** | 58.64 | 52.02+/-2.58 | 46.46+/-0.87 | 39.49+/-2.75 |
ITS1-F | 51.04 | 42.31+/-1.2 | 38.91+/-2.62 | 31.64+/-0.67 |
ITS2 | 56.68 | 48.5+/-1.97 | 39.3+/-2.74 | 32.99+/-5.67 |
ITS5 | 51.64 | 41.8+/-1.69 | 36.6+/-3.93 | NA |
ITS3 | 56.68 | 50.6+/-1.15 | 44.3+/-3.65 | 39.93+/-7.25 |
ITS4 | 50.9 | 45.04+/-1.3 | 35.94+/-3.38 | 32.73+/-1.83 |
ITS4-B | 59.33 | 54.49+/-2.39 | 46.6+/-3.06 | 37.72+/-7.38 |