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Table 2 Percentage of sequences amplified in silico, allowing one mismatch, from ascomycetes, basidiomycetes and 'non-Dikarya' with different primer combinations and using the three sequence subsets 1-3 (see Material and Methods) as templates.

From: ITS as an environmental DNA barcode for fungi: an in silico approach reveals potential PCR biases

Data subsets

Primer comb.

Ascomycetes

Basidiomycetes

'non-Dikarya'

Subset 1

ITS1*-ITS2

61.21

86.21

88.57

 

ITS1-F*-ITS2

90.75

99.14

92.38

 

ITS5*-ITS2

90.84

99.14

98.10

Subset 2

ITS1*-ITS4

61.91

82.00

84.86

 

ITS3*-ITS4

98.39

73.91

91.04

 

ITS5-ITS2*

94.89

72.10

92.63

Subset 3

ITS3-ITS4*

94.71

85.55

98.49

 

ITS3-ITS4-B*

-

39.31

-

  1. * primer evaluated for mismatches within each pair