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Figure 5 | BMC Microbiology

Figure 5

From: Gene encoding γ-carbonic anhydrase is cotranscribed with argC and induced in response to stationary phase and high CO2 in Azospirillum brasilense Sp7

Figure 5

Determination of argC / gca1 transcription unit and transcription start site of argC/gca1 transcript. A. Agarose gel showing amplified products obtained by reverse transcriptasepolymerase chain reaction (RT-PCR) with total RNA isolated from Azospirillum brasilense Sp7 using argF/argR1 (Lane 3), gcaF1/gcaR1 (Lane 4) and argF1/gcaR3 (Lane 5) primer sets. Lane 1 and 2 shows the bands of 100 bp DNA ladder (NEB) and control without reverse transcriptase, respectively; B. Determination of argC/gca1 transcription start site by 5' RACE experiment. The electropherogram is representative of results from sequencing of several distinct clones obtained after 5'RACE. The first base (C) downstream of the dT tail-A corresponds to the first nucleotide transcribed or TSS; C. Schematic representation of the argC-gca1 chromosomal region of A. brasilense. Large arrows represent the ORFs, and their orientation shows the transcriptional direction. Small arrows indicate the location of primers used for RT-PCR and 5'RACE experiment. The nucleotides representing TSS (+1), putative -35 and -10 boxes, and SD are underlined. Start codon (ATG) of argC is italicized.

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BMC Microbiology

ISSN: 1471-2180

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