Figure 4

Biodistribution of Bac7(1-35)-Alexa680 in healthy mice after i.p. injection. (A) The animal was placed in prone position, fluorescence emission in regions of interest encompassing the kidneys were acquired at indicated times post-injection and normalized. (B) The animal was placed in supine position, fluorescence emission in regions of interest encompassing the thorax and abdomen was acquired at indicated times post-injection and normalized. (C) Ex vivo images of organs at 5 hours after i.p. injection. Imaging of the organs was performed immediately after sacrifice: laser power and integration time were optimized while keeping constant scan step to compare fluorescence intensities after normalization. The images are representative of two independent experiments with comparable results.