Rapid and sensitive detection of Citrus Bacterial Canker by loop-mediated isothermal amplification combined with simple visual evaluation methods

Table 2 CBC-LAMP assay sensitivity from pure DNA

Detection method	Purified *Xanthomonas citri subsp.* citri DNA
	100 ng	10 ng	1 ng	100 pg	10 pg	1 pg	100 fg	10 fg	1 fg
Gel	+	+	+	+	+	+	+	+	-
LFD	+	+	+	+	+	+	+	+	-
SYBRGreen	+	+	+	+	+	+	Nc	Nc	-

For each dilution the CBC-LAMP reaction was performed in triplicate. Gel: gel electrophoresis. LFD: lateral flow dipstick.⁺: Positive reaction.^-: Negative reaction. Nc: The colour developed in the test tube was not clearly distinguishable between a positive or negative reaction.

ISSN: 1471-2180