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Table 1 Strains and plasmids used in this study

From: Bicarbonate enhances expression of the endocarditis and biofilm associated pilus locus, ebpR-ebpABC, in Enterococcus faecalis

Strain or Plasmid

Relevant characteristics

Source or reference

E. coli strains

  

   TG1

E. coli general cloning host

[35]

E. faecalis strains

  

   OG1RF

E. faecalis. FusR, RifR

[36]

   TX5266

OG1RF fsrB deletion mutant, deletion from bp 79 to 684 of fsrB. FusR, RifR

[6]

   TX5514

OG1RF ebpR deletion mutant, deletion from -5 bp to +1337 bp of ebpR. FusR, RifR

[11]

   TX5584

TX5514(pMSP3535). ErmR, FusR, RifR

[11]

   TX5582

TX5514(pTEX5515); ebpR mutant containing ebpR gene cloned into pMSP3535. ErmR, FusR , RifR

[11]

   TX5583

OG1RF(pMSP3535). ErmR, FusR, RifR

This study

   TX5581

OG1RF(pTEX5515); ebpR mutant containing ebpR gene cloned into pMSP3535. ErmR, FusR , RifR

This study

Plasmids

  

   pTCV-lacZ

Shuttle vector containing promoterless lacZ. ErmR

[32]

   pMSP3535

Nisin inducible expression shuttle vector with pAMβ 1 and ColE1 replicons. ErmR

[37]

   pTEX5269

fsrB promoter cloned upstream of lacZ in pTCV-lacZ (P fsrB ::lacZ), from bp -110 to -8 (103 bp) relative to fsrB start codon; ErmR

[6]

   pTEX5585

ebpA promoter cloned upstream of lacZ in pTCV-lacZ (P ebpA ::lacZ), from -221 bp to +80 bp (301 bp) relative to ebpA start codon. ErmR

This study

   pTEX5586

ebpR promoter cloned upstream of lacZ in pTCV-lacZ (P ebpR ::lacZ), from -248 to + 53 bp (301 bp) relative to ebpR start codon. ErmR

[11]

   pTEX5515

pMSP3535 with ebpR from -20 bp to +1561 bp from the ATG. This ebpR fragment contains the full ORF and the RBS of ebpR. ErmR

[11]