Strain or Plasmid | Relevant characteristics | Source or reference |
---|---|---|
E. coli strains | Â | Â |
   TG1 | E. coli general cloning host | [35] |
E. faecalis strains | Â | Â |
   OG1RF | E. faecalis. FusR, RifR | [36] |
   TX5266 | OG1RF fsrB deletion mutant, deletion from bp 79 to 684 of fsrB. FusR, RifR | [6] |
   TX5514 | OG1RF ebpR deletion mutant, deletion from -5 bp to +1337 bp of ebpR. FusR, RifR | [11] |
   TX5584 | TX5514(pMSP3535). ErmR, FusR, RifR | [11] |
   TX5582 | TX5514(pTEX5515); ebpR mutant containing ebpR gene cloned into pMSP3535. ErmR, FusR , RifR | [11] |
   TX5583 | OG1RF(pMSP3535). ErmR, FusR, RifR | This study |
   TX5581 | OG1RF(pTEX5515); ebpR mutant containing ebpR gene cloned into pMSP3535. ErmR, FusR , RifR | This study |
Plasmids | Â | Â |
   pTCV-lacZ | Shuttle vector containing promoterless lacZ. ErmR | [32] |
   pMSP3535 | Nisin inducible expression shuttle vector with pAMβ 1 and ColE1 replicons. ErmR | [37] |
   pTEX5269 | fsrB promoter cloned upstream of lacZ in pTCV-lacZ (P fsrB ::lacZ), from bp -110 to -8 (103 bp) relative to fsrB start codon; ErmR | [6] |
   pTEX5585 | ebpA promoter cloned upstream of lacZ in pTCV-lacZ (P ebpA ::lacZ), from -221 bp to +80 bp (301 bp) relative to ebpA start codon. ErmR | This study |
   pTEX5586 | ebpR promoter cloned upstream of lacZ in pTCV-lacZ (P ebpR ::lacZ), from -248 to + 53 bp (301 bp) relative to ebpR start codon. ErmR | [11] |
   pTEX5515 | pMSP3535 with ebpR from -20 bp to +1561 bp from the ATG. This ebpR fragment contains the full ORF and the RBS of ebpR. ErmR | [11] |