Skip to main content

Table 2 Robust performance of standard control genes using CAB as sole reference to set a manual threshold at 26 Ct and a master equation derived from 80 replicated plate reactions on Applied Biosystems 7500 real time PCR System

From: Quantitative transcription dynamic analysis reveals candidate genes and key regulators for ethanol tolerance in Saccharomyces cerevisiae

Control gene

Reference Ct

Mean Ct

Stdev

Estimated mRNA (pg)

Input mRNA (pg)

Consistency (%)

MSG

 

29.429

0.077

0.098

0.1

98.1

CAB

26.0

25.965

0.037

0.984

1

98.4

RBS1

 

22.388

0.019

10.64

10

93.6

ACTB

 

15.604

0.019

973.25

1000

97.3