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Table 4 Genetic loci transcripts significantly altered between 16M and 16MΔvjbR, with or without the treatment of C12-HSL that may contribute to virulence.

From: Brucella melitensis VjbR and C12-HSL regulons: contributions of the N-dodecanoyl homoserine lactone signaling molecule and LuxR homologue VjbR to gene expression

BME Loci

Gene Function

Exponential Growth Phase Change (fold)

Stationary Growth Phase Change (fold)

STM

  

Δ vjbR /wt

wt + AHL/wt

Δ vjbR /Δ vjbR + AHL

Δ vjbR /wt

wt + AHL/wt

Δ vjbR /Δ vjbR + AHL

 

Cell Membrane

I 1873

Autotransporter Adhesin

-2.2

-

-

-

-

-

 

II 1069

Adhesin, AidA

-1.5†

-

-

-1.5

-

-

 

I 0402

31 KDa OMP Precursor

-

1.5†

-

-1.7

-1.7†

-

 

I 0330

OpgC Protein

-

-2.0

-1.9†

-

-

-

 

I 0671

Integral Membrane Protein, Hemolysin

-

-2.7

-2.2†

-

-

-

[28]

II 1070

Adhesin AidA-I

1.7

-

-

-

-

-1.9†

 

I 1304

Porin, F Precursor

-

-

-3.6†

-3.5

-2.0

-2.6†

 

I 1305

Porin

-

-2.3

-1.8†

-1.5†

-

-

 

Cell Motility

II 0151

Flagellar M-Ring Protein, FliF

-2.2

-2.1

-

2.1†

-

-

[22, 34]

II 0161

Flagellar Hook-Associated Protein 3

-1.8†

-2.7

-

-

-

-

 

II 0165

Flagellar Biosynthesis Protein

-1.9†

-2.8

-

-

-

-

 

I 1692

Flagellar Protein, FlgJ

-

-

-2.3†

-1.8

-2.1

-3.4†

 

II 0160

Flagellar Hook-Associated Protein, FlgK

-1.6†

-2.0

-1.7†

-

-

-

 

II 0162

FlaF Protein

-2.1

-2.0†

-

-

-

-1.6†

 

II 0167

Flagellar Biosynthesis Protein, FlhA

-1.6†

-2.3

-1.8†

-1.5†

-1.9†

-5.5†

 

II 1109

Chemotaxis Protein, MotA

-1.6†

2.0†

-3.6†

-1.7

-1.5†

-

 

Protease and Lipoprotein

I 0611

HflC Protein, Stomatin, Prohibitin, Flotillin, HflK-C Domains

-1.6

-

-

-

-1.7†

-

 

I 1079

Lipoprotein NlpD

-

-1.5†

-1.6†

-1.6†

-1.9

-

 

I 1799

Lipoprotein Signal Peptidase

2.2

2.1†

-

-

-1.6†

-

 

II 0831

Hypothetical Protein, Aminopeptidase-Like Domain

-1.6†

-2.0

-

-2.3

-

3.1†

 

I 0213

Metalloendopeptidase

-1.7†

-2.7†

-1.6†

2.1

-

-

 

I 0282

Zinc Metalloprotease

-1.8

-1.7

-

-

-

3.4†

 

II 0149

Extracellular Serine Protease

-3.2

-1.8

2.9†

-

-1.7

-

 

Secretion System

I 0390

VceA

-1.4†

-1.3†

-

-

-1.2†

-

 

I 0948

VceC

1.1†

1.4†

-

1.6†

1.3†

-

 

I 1094

Exopolysaccharide Production Negative Regulator Precursor, Tetratricopeptide Repeat

-

-

-

2.1

1.5†

-

 

I 1141

Predicted Exported Protein

-1.6

-1.7

-

-

-

-

 

I 1531

Tetratricopeptide Repeat Family Protein

-2.1

-2.4

-

-1.7

-

-

[34]

I 1077

Hypothetical Exported Protein, YajC

-1.5

-2.1

-

-1.8†

-1.5†

1.8†

 

II 0025

Attachment Mediating Protein VirB1

-2.2

-1.9

-

-2.6

-2.2

-

[29, 31, 36]

II 0026

Attachment Mediating Protein VirB2

-

-2.1

-

-4.3

-3.6

-1.3†

[29, 31, 36]

II 0027

Channel Protein VirB3

-

-

-

-3.9

-3.2

-

[29–31, 36]

II 0029

Attachment Mediating Protein VirB5

-2.0

-

1.6†

-5.7

-4.5

-1.2†

[29–32]

II 0030

Channel Protein VirB6

-

-

-1.7†

-2.8

-2.3

-

[29–31, 36]

II 0032

Channel Protein VirB8

-1.6†

-

1.1†

-3.3

-2.6

-

[29, 31, 32, 36]

II 0033

Channel Protein VirB9

-

-

-

-1.8

-1.9

-

[29, 31, 36]

II 0034

Channel Protein VirB10

-

-1.5

-

-2.0

-1.9

-

[29, 31, 36]

II 0036

OMP, OprF, VirB12

-

-

-

-1.7

-1.7

-

[29, 36]

II 0466

Tetratricopeptide Repeat Family Protein

-

2.3

2.2†

-1.5†

-

-

 

Signal Transduction

II 0011

Transcriptional Regulatory Protein, HydG

-1.5†

-2.0

-

-

-

-

[31]

II 1014

Two Component Response Regulator

-

1.7†

-

1.6

-1.5†

-

 

I 0370

Sensory Transduction Histidine Kinase

-1.7

-2.1

-2.2†

-1.6†

-

2.1†

 

I 0372

Two-Component Response Regulator

1.6†

-

-1.5†

1.5†

1.8

-

 

I 2034

Sensor Protein, ChvG

-

-1.7

-2.4†

-2.0

-1.6

-

 

Stress Response

I 0887

Peptidyl-Prolyl Cis-Trans Isomerase

-

-1.7

-

1.7

1.6

-

 

I 1619

Hsp33-Like Chaperonin

-

-

-

1.8

1.6†

-

 

II 0245

Universal Stress Protein Family, UspA

-1.8

-1.7

-2.0†

-2.5

-2.5

-

 
  1. A (-) indicates genes excluded for technical reasons or had a fold change of less than 1.5; † genes that did not pass the statistical significance test but showed an average alteration of at least 1.5-fold. Fold change values are the averaged log2 ratio of normalized signal values from two independent statistical analyses. Abbreviations are as follows: STM, Signature Tagged Mutagenesis; OMP, Outer Membrane Protein.