Brucella melitensis VjbR and C12-HSL regulons: contributions of the N-dodecanoyl homoserine lactone signaling molecule and LuxR homologue VjbR to gene expression

Table 2 Transcripts associated with gene regulation significantly altered between 16M and 16MΔvjbR, with and without the treatment of C₁₂-HSL to cells.

BME Loci	Gene Function	Exponential Growth Phase Change (fold)			Stationary Growth Phase Change (fold)			STM
		Δ vjbR /wt	wt+AHL/wt	Δ vjbR /Δ vjbR +AHL	Δ vjbR /wt	wt+AHL/wt	Δ vjbR /Δ vjbR +AHL
I 0019	LacI Family	-2.9	-1.8^†	-	1.9	1.5^†	-
I 0305	DeoR Family	-1.7	-1.7^†	-	1.9	1.5^†	-	[31]
I 0447	Leucine-Responsive Regulatory Protein	1.6	-	-	-2.4	-1.8	-
I 0781	DNA-Directed RNA Polymerase A Subunit	2.4^†	2.8	-	-	-	-	[34]
I 1383	AraC Family	-2.4	-1.5^†	-	-	-1.7^†	-
I 1607	LuxR Family DNA Binding Domain	1.8^†	3.0	-	-1.5^†	-	-
I 1631	TetR Family	-1.9	-2.1	-	-	-	-
I 1700	Predicted Transcriptional Regulator	2.0	2.9	-	-	-	-
II 0051	LuxR Family DNA Binding Domain	-1.9	-2.8	-	-	-	-
II 0800	AraC Family	1.7	2.2^†	-	-	-	-
II 0854	CRP Family Transcriptional Regulator	-	1.6^†	-	-1.5	-1.7	-
II 0985	LacI Family	-2.5	-2.7^†	-	-2.4	-	-
II 1022	IclR Family	-1.5^†	-1.8	-	-1.9	-2.1	-
II 1098	AraC Family	-1.8	-2.8	-	1.9	1.5	-
I 0446	MarR Family	1.9^†	2.9	2.9^†	-	-	-
I 0518	Cold Shock Protein, CspA	1.6	-	-2.0^†	1.7	-	-
I 0720	Sugar Fermentation Stimulation Protein	-	-2.0	1.7^†	-1.7^†	-	1.5^†
I 0899	Phage-Related DNA Binding Protein	-1.8	-1.5^†	-1.9^†	1.6	-	-2.4^†
I 1098	AsnC Family	-1.7	-2.0	-1.6^†	-1.6	-	-
I 1291	AraC Family	-	-1.9	-1.7^†	1.7	-	-
I 1641	TetR Family	-	-	-2.7^†	-1.7	-1.8	-
I 1885	LysR Family	-	-1.8^†	-2.3^†	-1.6	-	-
II 0127	IclR Family	-	1.6^†	-	-1.8	-	1.6^†
II 0219	IclR Family	-3.2	-5.8	-3.8^†	-1.5^†	-	-
II 0657	Transcription Elongation Factor	2.4^†	3.1	-	-	-	2.4^†
II 0810	ArsR Family	-	2.0	-	1.8	1.6^†	-2.3^†

A (-) indicates genes excluded for technical reasons or had a fold change of less than 1.5; ^† genes that did not pass the statistical significance test but showed an average alteration of at least 1.5-fold. Fold change values are the averaged log₂ ratio of normalized signal values from two independent statistical analyses. Abbreviations as follows: STM, Signature Tagged Mutagenesis.

ISSN: 1471-2180