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Table 2 Quantitative analysis of the expression of SE2472 proteins upon exposure to H2O2.

From: Mass spectrometry-based quantitative proteomic analysis of Salmonella enterica serovar Enteritidis protein expression upon exposure to hydrogen peroxide

Locus Tag

Description

Gene

% Change

PSLT011

Dlp (SrgA)

srgA

12 ± 2%

STM0007

Transaldolase B

talB

0%

STM0012

Chaperone protein dnaK (Heat shock protein 70)

dnaK

56 ± 7%

STM0013

Chaperone protein dnaJ

dnaJ

38 ± 3%

STM0093

Organic solvent tolerance protein

Imp

210 ± 30%

STM0102

L-arabinose isomerase

araA

26 ± 2%

STM0158

Bifunctional aconitate hydratase

acnB

25 ± 5%

STM0217

Elongation factor Ts

tsf

21 ± 4%

STM0316

Aminoacyl-histidine dipeptidase

pepD

9 ± 1%

STM0432

Phosphonoacetaldehyde hydrolase

phnX

31 ± 3%

STM0435

Nucleotide-binding protein

yajQ

0%

STM0447

Trigger factor

tig

11 ± 2%

STM0488

Adenylate kinase

adk

0%

STM0536

Peptidyl-prolyl cis-trans isomerase B

ppiB

0%

STM0608

Chain T, crystal structure of Ahpc

ahpC

0%

STM0730

Citrate synthase

gltA

42 ± 5%

STM0772

Phosphoglyceromutase

gpmA

-40 ± 10%

STM0776

UDP-galactose 4-epimerase

galE

23 ± 2%

STM0781

Molybdate transporter periplasmic protein

modA

11 ± 2%

STM0794

Biotin synthase

bioB

0%

STM0830

Glutamine-binding periplasmic protein precursor

glnH

10 ± 3%

STM0877

Putrescine-binding periplasmic protein precursor

potF

11 ± 2%

STM0999

Outer membrane protein F precursor

ompF

0%

STM1091

Secretory Effector Protein

SopB

-55% ± 7%

STM1220

N-acetyl-D-glucosamine kinase

nagK

12 ± 3%

STM1231

DNA-binding response regulator in PhoQ system

phoP

20 ± 6%

STM1290

Glyceraldehyde-3-phosphate dehydrogenase

gapA

31 ± 3%

STM1296

Putative oxidoreductase

ydjA

-30 ± 5%

STM1302

Exonuclease III

xthA

0%

STM1303

Succinylornithine transaminase

astC

41 ± 7%

STM1310

NAD synthetase

nadE

9 ± 1%

STM1378

Pyruvate kinase I

pykF

87 ± 12%

STM1431

Superoxide dismutase

sodB

110 ± 20%

STM1544

PhoPQ-regulated protein

pqaA

19 ± 2%

STM1567

Alcohol dehydrogenase

adhP

9 ± 2%

STM1589

Putative NADP-dependent oxidoreductase

yncB

12 ± 2%

STM1641

ATP-dependent helicase

hrpA

20 ± 3%

STM1661

Putative universal stress protein

ydaA

140 ± 20%

STM1682

Probable peroxidase

tpx

19 ± 2%

STM1714

DNA topoisomerase I

topA

17 ± 4%

STM1727

Tryptophan synthase

trpA

37 ± 9%

STM1746.S

Chain A, structural basis of multispecificity in Oppa

oppA

0%

STM1796

Trehalase, periplasmic

treA

25 ± 3%

STM1886

Glucose-6-phosphate 1-dehydrogenase

zwf

0%

STM1923

Chemotaxis protein motA

motA

14 ± 3%

STM1954

Cystine-binding periplasmic protein precursor

fliY

9 ± 2%

STM1959

Flagellin

fliC

0%

STM2104

Phosphomannomutase in colanic acid gene cluster

cpsG

23 ± 6%

STM2167

NADH independent D-lactate dehydrogenase

dld

16 ± 5%

STM2190

D-galactose binding periplasmic protein

mglB

34 ± 3%

STM2203

Endonuclease IV

nfo

0%

STM2205

Fructose-1-phosphate kinase

fruK

35 ± 3%

STM2282

Glycerophosphodiester phosphodiesterase

glpQ

15 ± 3%

STM2337

Acetate kinase

ackA

23 ± 3%

STM2347

Putative phosphoesterase

yfcE

0%

STM2362

Amidophosphoribosyltransferase

purF

10 ± 4%

STM2501

Polyphosphate kinase

ppk

7 ± 3%

STM2549

Anaerobic sulfide reductase

asrB

0%

STM2647

Uracil-DNA glycosylase

ung

27 ± 2%

STM2829

DNA strand exchange and recombinant protein

recA

24 ± 2%

STM2864

Iron transporter protein, fur regulated

sitD

-45 ± 8%

STM2882

Secretory Effector Protein

sipA

0%

STM2884

Translocation Machinery Component

sipC

301 ± 30%

STM2924

RNA polymerase sigma factor rpoS

rpoS

13 ± 2%

STM2952

Enolase

eno

23 ± 4%

STM2976

L-fucose isomerase

fucI

0%

STM2993

Exonuclease V, alpha chain

recD

0%

STM3068

Fructose-bisphosphate aldolase

fba

52 ± 7%

STM3069

Phosphoglycerate kinase

pgk

20 ± 3%

STM3186

Outer membrane channel protein

tolC

0%

STM3219

2,4-dieonyl-CoA reductase

fadH

24 ± 3%

STM3225

Serine/threonine transporter

sstT

23 ± 3%

STM3294

Phosphoglucosamine mutase

glmM

18 ± 2%

STM3342

Stringent starvation protein A

sspA

-20 ± 4%

STM3359

Malate dehydrogenase

mdh

36 ± 6%

STM3380

Acetyl CoA carboxylase

accC

11 ± 2%

STM3401

Shikimate dehydrogenase

aroE

12 ± 3%

STM3445

Elongation factor Tu

tuf

0%

STM3446

Elongation factor G

fusA

9 ± 2%

STM3484

DNA adenine methylase

dam

26 ± 3%

STM3496

Putative hydrolase

yrfG

0%

STM3500

Phosphoenolpyruvate carboxykinase

pckA

330 ± 40%

STM3502

Osmolarity response regulator

ompR

12 ± 3%

STM3557

Glycerol-3-phosphatase transporter binding protein

ugpB

0%

STM3612

2-dehydro-3-deoxygluconokinase

kdgK

9 ± 2%

STM3884

D-ribose periplasmic binding protein

rbsB

31 ± 3%

STM3968

Uridine phosphorylase

udp

11 ± 5%

STM3997

Thiol:disulfide interchange protein

dsbA

10 ± 5%

STM4029

Putative acetyltransferase

yiiD

0%

STM4166

NADH pyrophosphatase

nudC

10 ± 2%

STM4256

Single-strand DNA-binding protein

ssb

19 ± 2%

STM4329

Co-chaperonin groES

groES

51 ± 3%

STM4330

Chaperonin groEL

groEL

43 ± 2%

STM4343

Fumarate reductase

frdA

40 ± 2%

STM4359

DNA mismatch repair protein mutL

mutL

41 ± 3%

STM4414

Inorganic pyrophosphatase

ppa

0%

STM4513

Putative permease

yjiG

-78 ± 15%

STM4567

Deoxyribose-phosphate aldolase

deoC

0%

STM4568

Thymidine phosphorylase

deoA

-9 ± 2%

STM4569

Phosphopentomutase

deoB

0%

STM4598

Two-component response regulator

arcA

10 ± 4%

STY2300

CDP-6-deoxy-D-xylo-4-hexulose-3-dehydrase

rfbH

0%

STY2300

CDP-4-keto-6-deoxy-D-glucose-3-dehydrase

ddhC

0%

  1. Relative expression level in the presence of H2O2 compared against control (in the absence of H2O2) is shown. An average of 10 peaks was used to calculate the mean intensity ratios and the error percentage of each protein spot. The results were the average from triplicate experiments. The limit of detection was arbitrarily set to 5% and any proteins that showed less than 5% change were classified as 0%.