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Figure 6 | BMC Microbiology

Figure 6

From: Fibronectin-binding protein B variation in Staphylococcus aureus

Figure 6

Binding of polyclonal and monoclonal anti-isotype I A domain antibodies to recombinant A domain isotypes I - VII. Microtitre dishes were coated with A domains isoype I - VII at the indicated concentrations. Wells were blocked and then incubated with (a) polyclonal rabbit anti-isotype I A domain antibodies, or (b) mouse monoclonal anti-isotype I A domain antibody 2E11. Bound antibodies were detected with either (a) HRP-conjugated goat anti-rabbit IgG antibodies or (b) HRP-conjugated goat anti-mouse IgG antibodies followed by TMB substrate. Graphs are representative of three separate experiments.

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