Mycobacterium tuberculosis Rv0679c protein sequences involved in host-cell infection: Potential TB vaccine candidate antigen
© Cifuentes et al; licensee BioMed Central Ltd. 2010
Received: 24 September 2009
Accepted: 13 April 2010
Published: 13 April 2010
To date, the function of many hypothetical membrane proteins of Mycobacterium tuberculosis is still unknown and their involvement in pathogen-host interactions has not been yet clearly defined. In this study, the biological activity of peptides derived from the hypothetical membrane protein Rv0679c of M. tuberculosis and their involvement in pathogen-host interactions was assessed. Transcription of the Rv0679c gene was studied in 26 Mycobacterium spp. Strains. Antibodies raised against putative B-cell epitopes of Rv0679c were used in Western blot and immunoelectron microscopy assays. Synthetic peptides spanning the entire length of the protein were tested for their ability to bind to A549 and U937 cells. High-activity binding peptides (HABPs) identified in Rv0679c were tested for their ability to inhibit mycobacterial invasion into cells.
The gene encoding Rv0679c was detected in all strains of the M. tuberculosis complex (MTC), but was only transcribed in M. tuberculosis H37Rv, M. tuberculosis H37Ra and M. africanum. Anti-Rv0679c antibodies specifically recognized the protein in M. tuberculosis H37Rv sonicate and showed its localization on mycobacterial surface. Four HABPs inhibited invasion of M. tuberculosis to target cells by up to 75%.
The results indicate that Rv0679c HABPs and in particular HABP 30979 could be playing an important role during M. tuberculosis invasion of host cells, and therefore could be interesting research targets for studies aimed at developing strategies to control tuberculosis.
Tuberculosis (TB) is among the top three leading causes of death by a single infectious agent worldwide. The situation is further aggravated by the increased susceptibility of human immunodeficiency virus (HIV)-positive people to infection with Mycobacterium tuberculosis, and by the emergence of multidrug-resistant (MDR)-TB strains in many geographical areas . An estimate of nearly 9.2 million cases of TB occurred during 2007, 4.1 million of which corresponded to new smear-positive cases and 14.8% were reported among HIV-positive people .
Unfortunately, the bacillus Calmette-Guérin (BCG) vaccine is insufficient to control the worldwide spread of this health threat, especially since it is contraindicated for HIV-positive people and has a variable efficacy, mostly due to its low capacity to stimulate the broad cell spectrum needed for inducing an effective immune response [4, 5]. Therefore, a large body of research has focused on searching for new specific antigens of M. tuberculosis that could be used as new prophylactic alternatives with the aim of replacing or improving the currently available BCG vaccine [6–8].
The publication of the complete M. tuberculosis H37Rv genome sequence has opened a gate for the identification of genes that encode M. tuberculosis antigens putatively able to trigger an effective immune response and that could therefore be interesting as potential components of antituberculous subunit vaccines [9, 10]. The immunological properties of these predicted M. tuberculosis-specific antigens have been characterized mainly using recombinant proteins . Synthetic peptides have been also used with success for screening pathogen-specific genome regions of putative protective importance in order to identify T-cell reactivity . In TB, synthetic peptides have shown good results for diagnosing TB in cattle  and in a protective vaccine tested in mice .
The first encounter between M. tuberculosis and the host cell occurs via an array of different receptor molecules, including complement receptors, the mannose receptor, the dendritic cell-specific intercellular adhesion molecule (ICAM)-3-grabbing nonintegrin (DC-SIGN), and Fc receptors . The recent discovery of novel classes of receptors such as toll-like receptors, nucleotide-binding oligomerization domain (NOD)-like receptors, DC-SIGN, and Dectin-1, are giving clues about the possible host mechanisms involved in coordinating the innate and adaptive immune responses against M. tuberculosis. Particularly, lipoproteins have been shown to trigger cytokine signaling via toll-like receptors on the surface of mammalian cells and therefore have been considered to be important effectors that may contribute to the pathogen's virulence. However, only a reduced number of predicted mycobacteriallipoproteins have been experimentally characterized .
Our institute has studied ligand-receptor interactions established between synthetic peptides derived from pathogen proteins and host-cell surface receptors, with the purpose of identifying high activity binding peptides (HABPs) involved in specific host-pathogen recognition interactions, and that could therefore be potential components of subunit vaccines. This methodology has been used and tested on different pathogens, including Plasmodium falciparum, Plasmodium vivax[18–20], Human papillomavirus  and Epstein-Barr virus , among others. Specifically in the case of M. tuberculosis, our group has characterized and determined the binding profiles of three mycobacterial membrane proteins [23–25]. More recently, the biological relevance of HABPs derived from some other mycobacterial proteins has been demonstrated using a flow-cytometry-based assay to assess the capacity of HABPs to mycobacterial inhibit invasion of target cells [26–28].
This study focused on the Rv0679c protein of M. tuberculosis, which is classified as a hypothetical membrane protein of the cell envelope. Its protein homolog in M. bovis BCG is a putative lipoprotein that has been shown to be tightly associated to lipoarabinomannan (LAM) , one of the major components of cell envelope involved in pro-inflammatory and anti-inflammatory responses . The aim of the present study was to identify Rv0679c HABPs capable of inhibiting M. tuberculosis invasion of target cells that could therefore be considered as potential as candidate components for a chemically synthesized, subunit-based antituberculous vaccine.
The sequence of the M. tuberculosis Rv0679c protein was downloaded from Tuberculist http://genolist.pasteur.fr/TubercuList/ and used as query sequence of a BLAST search http://0-www.ncbi.nlm.nih.gov.brum.beds.ac.uk/BLAST/. Type I and II signal peptides (typical of lipoproteins) were identified using LipoP 1.0 http://www.cbs.dtu.dk/services/LipoP/. Transmembrane regions were predicted using TMHMM v. 2.0 http://www.cbs.dtu.dk/services/TMHMM and TMPRED http://www.ch.embnet.org/software/TMPRED_form.html.
The presence and transcription of the Rv0679c gene was assessed in species and strains belonging to the M. tuberculosis complex and in mycobacteria other than tuberculosis. The following strains were tested (26 in total): M. tuberculosis H37Rv (ATCC 27294), M. tuberculosis H37Ra (ATCC 25177), M. bovis (ATCC 19210), M. bovis BCG (ATCC 35734), M. africanum (ATCC 25420), M. microti strain Pasteur (donated by Dr. Françoise Portaels), M. flavescens (ATCC 14474), M. fortuitum (ATCC 6841), M. szulgai (ATCC 35799), M. peregrinum (ATCC 14467), M. phlei (ATCC 11758), M. scrofulaceum (ATCC 19981), M. avium (ATCC 25291), M. smegmatis (ATCC 14468), M. nonchromogenicum (ATCC 19530), M. simiae (TMC 1595), M. intracellulare (ATCC 13950), M. gastri (ATCC 15754), M. kansasii (ATCC 12478), M. dierhoferi (ATCC 19340), M. gordonae (ATCC 14470), M. marinum (ATCC 927), M. terrae (ATCC 15755), M. chelonae-chelonae (ATCC 35752), M. vaccae (ATCC 15483), M. triviale (ATCC 23292). All mycobacterial strains were cultured for 5 to 15 days in Middlebrook 7H9 medium (Difco, New Jersey, USA) containing 0.05% Tween 80. Growth media were supplemented with oleic acid-albumin-dextrose-catalase (OADC) (Becton Dickinson, BBL; Sparks, MD) or ADC as needed. Genomic DNA isolated phenol-chloroform extraction, as described elsewhere . PCR assays were carried out on a GeneAmp PCR System 9600 thermal cycler (Perkin-Elmer Life Sciences Inc., Boston, MA, USA) using 0.4 mM of direct (5'-CGCTACCCACTCCCG-3') and reverse primers (5'-CTTGTTGTTCGCACCAC-3') to amplify a 346-bp fragment of Rv0679c. Thermocycling conditions consisted of an initial denaturation at 94°C for 5 min, followed by 25 cycles according to the following conditions: 56°C for 30 s, 72°C for 40 s and 95°C for 40 s. A final 5 min extension step was performed at 72°C. Amplification products were separated in SYBR-stained 1% (w/v) agarose gels (Invitrogen).
Detection of Rv0679c by Western blot and immunoelectron microscopy (IEM)
Expression of the Rv0679c gene was assessed by Western blot analysis of M. tuberculosis H37Rv sonicates using sera raised in goats obtained. Briefly, two goats (A-29 and B-86) nonreactive to M. tuberculosis H37Rv sonicate were inoculated with 5 mg of either polymerized forms of peptide 28528 (43CGTTTPATATTTTATSGPTAAPGC62) or peptide 28530 (145CGTYKNGDPTIDNLGAGNRINKEGC165), both in polymeric form and emulsified with Freund's incomplete adjuvant. These two peptides were chosen because the BepiPred 1.0b server http://www.cbs.dtu.dk/services/BepiPred/ predicted them as B cell epitopes. Subcellular localization was determined in a CM 10 transmission electron microscope (Philips, Suresne, Hauts-de-Seine, France), using thin slices (400 nm) of LR-White resin embedded mycobacteria. Goat anti-peptide sera were used as primary antibody and anti-goat IgG coupled to 10-nm colloidal gold particles as secondary antibody. Slices were stained with 6% uranyl acetate to enhance image contrast.
Interaction of Rv0679c peptides with target cells
Nine nonoverlapping 20-mer-long peptides spanning the entire length of Rv0679c were synthesized and 125I-labeled according to previously described techniques [34–36]. Peptides were tested for their ability to bind to the A549 alveolar cell line (ATCC CLL-185) and to macrophages derived from U937 monocytes (ATCC CRL-2367). Briefly, 1.5 × 106 cells cultured in Roux flasks were dislodged using 1× Non-enzymatic Cell Dissociation Solution (Sigma) and incubated with increasing concentrations of 125I-labeled peptide (0-950 nM) in the presence or absence of unlabeled peptide (40 μM). Unbound peptide was removed using a dioctylphthalate-dibutylphthalate cushion, before measuring cell-associated radioactivity in a gamma counter (Gamma Counter Cobra II, Packard Instrument Co., Meriden, CT, USA).
Total binding minus nonspecific binding yielded the specific binding curve, whose slope corresponded to the binding activity of the peptide. Any peptide displaying a specific binding activity of ≥1% was considered a HABP [23–25, 37]. Binding constants were determined by performing a saturation assay using U937 cells and peptide concentrations larger than the ones used for binding assays (0-4500 nM).
Circular dichroism analyses of Rv0679c peptides
The secondary structure elements of the peptides spanning the entire length of Rv0679c were studied by circular dichroism. CD spectra of peptides (5 μM) dissolved in 30% trifluoroethanol (TFE) were acquired at 20°C by averaging three scans taken in a Jasco J-810 spectropolarimeter (wavelength range: 260-190 nm, scan rate: 20 nm/min, bandwidth: 1 nm), using a 1.00-cm pathway cuvette (Jasco Inc, Easton, MD). Data were corrected for baseline deviation . The results were expressed as mean residue ellipticity [θ], the units being degrees × cm2 × dmol-1 according to the [Θ] = Θλ/(100lcn) function, where θλ is the measured ellipticity, l is the optical path length, c is the peptide concentration, and n is the number of residues in the amino acid sequence.
Invasion inhibition assays
Rv0679c HABPs were assessed for their ability to inhibit mycobacterial invasion using a flow-cytometry-based assay developed by Bermúdez and Goodman  and later modified by us . In brief, A549 and U937 cells (1 × 106) seeded overnight on 6-well plates were incubated for 1 h with different peptide concentrations. SYBR-safe stained mycobacteria (10 × 106) suspended in RPMI medium were added to each well (MOI: 1:10) and incubated overnight at 37°C. Inhibition controls consisted of Cytochalasin D (3 μM) or colchicine (50 μM). Extracellular bacilli were first inactivated by incubation with Amikacin (200 μg/mL) for 1 h and then removed by successive washes with Hanks Balanced Salt Solution (HBSS). Cells were dislodged from monolayers and stained with methylene blue for FACscan flow cytometry analysis (Becton Dickinson). The percentage of SYBR safe positive events in the flanked region were determined by registering a total of 30 000 events (only infected cells were detected on the FL1 channel due to their fluorescence characteristics). Data were statistically analyzed by applying a student's t- test.
Internalization of latex beads
Internalization assays were carried out according to a methodology reported by El-Shazly and colleagues . Briefly, A549 cells (1 × 106) were exposed to peptide-coated fluorescent beads for 3 h. After removing noninternalized beads by washing cell thrice with HBSS, cells were dislodged from the monolayer and analyzed in a FACscan flow cytometer, same as described in invasion inhibition assays. The same assay was carried out using uncoated beads as negative control. An additional assay was carried out to determine whether the peptide alone enabled internalization of the latex beads by modifying the host cell membrane or whether internalization depended on the interaction between the peptide and the bead. For this assay, the control consisted on incubating cells for 2 h only with the peptide and then for 1 h with uncoated beads.
Molecular analysis of the Rv0679c gene
Goat anti-Rv0679c antibodies specifically recognized bands of about 18 and 20 kDa on M. tuberculosis sonicate and localized the protein on the surface
Binding of Rv0679c peptides to U937 and A549 cells
Rv0679c HABPs 30979 and 30987 were assessed by means of a saturation assay using concentrations of radiolabeled peptide larger than the ones used in conventional binding assays in order to determine dissociation constants (Kd), Hill coefficients (nH) and approximate number of binding sites per cell (Figure 4b). The results showed that binding of these HABPs to surface receptors of U937 cells was saturable and of cooperative nature (nH = 1.50 for HABP 30979 and nH = 1.12 for HABP 30987). A dissociation constant of 1,100 nM and about 1.0 × 106 binding sites per cell were identified for HABP 30979, while HABP 30987 showed a dissociation constant of 600 nM and about 1.8 × 106 binding sites per cell.
Secondary structure analyses of Rv0679c peptides by circular dichroism
Inhibition of M. tuberculosis H37Rv invasion into A549 and U937 cells
The ability of Rv0679c HABPs to block mycobacterial entrance into A549 and U937 cells was evaluated using a flow-cytometry-based assay. Rv0679c peptides analyzed in such assay included peptides 30979 and 30987, which had been identified as HABPs for both cell lines, peptides 30985 and 30986 which had been identified as HABPs for A549 cells, and a low activity binding peptide (30982) which was used as negative control. Invasion of U937 cells was significantly inhibited by HABPs 30985 and 30986, but neither of these two HABPs showed a clear dose-dependent inhibitory behavior. Peptides 30985 and 30986 showed some signs of cytotoxicity when they were used at the largest peptide concentration (200 μM), as indicated by the lost of a portion of the cell monolayer and an abrupt decrease in percentages of invasion inhibition. No other peptide showed cytotoxic effects.
Rv0679c HABPs 30986 and 30979 facilitate internalization of latex beads
A possible role for Rv0679c HABPs in host cell invasion was evaluated by determining their ability to facilitate internalization of fluorescent latex beads by A549 cells when beads are coated with these HABPs. Rv0679c peptides tested in this assay included 30979, 30985-30987, and peptide 30982 which was used as negative control. As it can be observed in Figure 6b, the highest internalization percentage was achieved when latex beads were coated with HABP 30979, followed by peptides 30985 and 30987. The percentage of internalization decreased when latex beads were coated with HABP 30986 compared to internalization of latex beads coated with the control peptide 30982. However, when cells were incubated first with each HABP and then with uncoated latex beads (control), smaller internalization percentages were found for all HABPs, and such percentages were smaller than the ones found when cells were incubated only with the beads (no peptide).
The mycobacterial cell envelope is a lipid-rich complex structure that surrounds the bacillus and is thought to play a critical role in the pathogenicity of Mycobacterium tuberculosis. Nearly 2.5% of the M. tuberculosis H37Rv proteome is predicted to consist of lipoproteins . A large number of these mycobacterial lipoproteins have been suggested to be important components for the synthesis of the mycobacterial cell envelope, as well as for sensing processes, protection from stressful factors and host-pathogen interactions; nevertheless, the function and localization of a considerable number of putative lipoproteins remains yet unknown .
Lipoproteins are translocated across the cytoplasmic membrane and then anchored to either the periplasm or the outer membrane and have been suggested to play important roles related to virulence because they are predicted to participate in intracellular transport, cell-wall metabolism, cell adhesion, signaling and protein degradation . Rv0679c was initially classified as a hypothetical membrane protein of M. tuberculosis and was later suggested to be a putative lipoprotein . It is a 165-amino-acid-long protein with a theoretical molecular mass of 16.6 kDa, whose function has not been fully characterized yet.
In this study, PCR and RT-PCR techniques were used to examine the distribution of the Rv0679c gene in the MTC, as well as in mycobacteria other than tuberculosis (which included saprophytic and environmental species), with the aim of establishing a preliminary relationship between the presence of the protein encoding gene in a particular mycobacterial species and its virulence, considering that to develop a subunit antituberculous vaccine, it would be better to select peptides (more specifically HABPs) from M. tuberculosis proteins involved in host cell invasion that are exclusively present in MTC or in mycobacterium species related to invasive processes or causing disease, such as Rv0679c. The results of this study indicate that the gene encoding Rv0679c is present in the MTC, as shown by the PCR amplification of a 346-bp band from genomic DNA of M. tuberculosis H37Rv, M. tuberculosis H37Ra, M. africanum, M. bovis, M. bovis BCG and M. microti; but no amplification was detected in Mycobacterium spp. strains outside the complex. Nevertheless, it is worth noting that Rv0679c homologues have been recently reported in different Mycobacterium genomes (e.g. M. smegmatis, M. marinum and M. avium), which indicates that such primers are specific for the MTC strains assessed in this study. Furthermore, reverse transcription assays indicate that the gene is actively transcribed in M. tuberculosis H37Rv, M. tuberculosis H37Ra and M. africanum. Intriguingly, although expression of Rv0679c homologous protein in M. bovis BCG was described by Matsuba et al. , gene transcription was not detected in M. bovis nor in M. bovis BCG in this study under normal culture conditions.
Once the presence and transcription of Rv0679c was determined in the MTC, the next step consisted in evaluating protein expression by Western blot analysis of M. tuberculosis H37Rv sonicate. Goat anti-Rv0679c peptide serum detected two bands of about 18 and 20 kDa, which differ from the theoretical molecular mass of 16.6 kDa predicted based on its amino acid composition. This slight difference could be caused by the post-translational modifications that lipoproteins undergo before reaching their destination as mature proteins, considering that pro-lipoproteins tend to be 2-3 kDa larger than mature lipoproteins .
According to bioinformatics predictions, Rv0679c lacks of transmembrane regions and contains an N-terminal signal sequence as well as a SPAse II cleavage site between residues 32-33, as indicated by the presence of a "lipobox" motif [LAGC] between amino acids 30-33. The presence of a signal peptide detected by using SignalP suggests that this protein is secreted via the Sec-dependent pathway, and is probably targeted by the lipobox motif to membrane surface where it remains attached by hydrophobic interactions. Briefly, after Rv0679c is translocated across the cytoplasmic membrane, the Cys residue of the lipobox motif is linked to a diacylglyceryl moiety. Then, a signal II peptidase cleaves off the signal peptide and the protein is anchored to the mycobacterial membrane via the diacylglyceryl moiety . These computational predictions are in agreement with the cellular localization observed in IEM studies in which the protein was detected on the surface of M. tuberculosi s H37Rv bacilli.
To determine whether the peptides comprising Rv0679c established ligand-receptor interactions with M. tuberculosi s susceptible human host cells, binding assays were performed with the U937 phagocytic and A549 epithelial cell lines. HABPs 30985 to 30987 comprising amino acids 121-165 showed higher binding activities to receptors on the surface of epithelial cells, whereas their binding activities to the phagocytic line were lower. Such differential binding behavior may be caused by differences between the surface receptors expressed by each cell line or their distinct physiological functions.
Interestingly, Rv0679c HABPs 30985, 30986 and 30987 are consecutively positioned within the protein's C-terminus, suggesting that the region formed by these three HABPs is implicated in binding of M. tuberculosis to target cells. Also, the Hill analysis showed high binding affinity interactions with a large number of receptor molecules on the surface of U937 cells, as indicated by their dissociation constant within the nanomolar range. Moreover, the formation of ligand-receptor complexes appears to facilitate binding of more HABPs, as shown by the positive Hill coefficient.
All HABPs tested in invasion inhibition assays prevented cell invasion by M. tuberculosis by a larger or comparable percentage, compared to the colchicine and Cytochalasin D controls. Regarding HABP 30986, an inhibitory effect similar to the one shown by HABPs 30985 and 30987 was observed on A549 cells at all concentrations used in this assay. Moreover, HABP 30987 showed larger inhibitory effect at the smaller concentration tested in this assay. HABP 30979 inhibited invasion of both cell types by a larger or even higher percentage than the ones shown by the colchicine and Cytochalasin controls. This HABP showed a dose-dependent inhibitory effect on both cells, achieving the highest inhibitory percentage at 200 μM.
The ability of Rv0679c peptides to inhibit M. tuberculosis invasion of target cells suggests that active and specific binding to cell surface receptors prevents entry of M. tuberculosis through this invasion pathway. Such notion is further supported by the results of internalization assays carried out with peptide-coated latex beads and epithelial cells, where peptide-coated beads were more actively internalized than uncoated beads. Particularly HABP 30979, which was the strongest invasion inhibitor, displayed the highest internalization percentages.
On the other hand, the large inhibition percentages obtained with phagocytic cells in comparison to the ones obtained with epithelial cells might be explained by the cooperativity phenomenon observed in saturation assays with the phagocytic cell line, since the amount of peptide that binds to surface receptors is proportional to the probability of forming more stable ligand-receptor complexes and thereby of restricting mycobacterial entrance. Furthermore, since some HABPs showed high binding activity to one cell type but low binding activity to the other one, it could be suggested that peptide binding activity depends on specific receptor molecules expressed on each cell type. Consequently, binding of Rv0679c HABPs with high activity to both cell lines could be due to the presence of the same receptor on both cell types or to different receptors with similar characteristics.
To date, no structural model has been reported for this protein. Therefore, CD assays were conducted in order to determine whether there was a relationship between the secondary structure of Rv0679c peptides and their binding ability or in their ability to inhibit mycobacterial invasion. CD spectrum data suggested that the secondary structure of HABP 30979 and 30985 was formed by α-helix and random coil elements, while peptides 30982 to 30984 and HABPs 30986 and 30987 showed undefined structural features. The results indicate that there is not a direct relationship between the structure of HABPs and their ability to binding to target cells.
Interestingly, the results obtained in this study showed that the HABPs that inhibited mycobacterial invasion to target cells more efficiently were also the ones that showed the larger internalization percentages, therefore suggesting that Rv0679c HABPs promote entry of pathogenic M. tuberculosis into host cells. Specifically, the binding region formed by HABPs 30985-30987 at the protein's C-terminal region appears to have a key role during M. tuberculosis invasion.
The confirmation of Rv0679c's location in mycobacterial surface, together with the identification of a binding region formed by HABPs 30985-30987, suggest that this protein may be related to adhesion and/or invasion processes. In addition, such surface localization could be facilitating contact between the bacilli and its host cell, thereby leading to triggering the host's immune response via interaction with host cell surface receptors .
The complexity of Mycobacterium tuberculosis as a pathogen and the variety of mechanisms that it uses for invading host cells makes it necessary to develop an effective strategy to block the invasion of target cells. Our proposal is based on searching for fragments of different proteins involved in the mycobacteria-host cell interaction. In our experience, sequences that bind specifically to target cells and that are capable of blocking invasion could be used as template to design peptides with ability to immunomodulate the protective response against tuberculosis. The immune response triggered against mycobacterial high-specific binding sequences could prevent invasion of target cells, either during a first encounter with the bacillum or during the reactivation of a latent infection.
It has been reported that a considerable number of secreted proteins are protective antigens and therefore have been considered as attractive candidates to develop subunit vaccines [43–46]. Moreover, they are hypothesized to mediate mycobacterial entry into the host cell .
Traditionally, vaccine development has been founded on the humoral immune response, which involves antibody production and is mainly targeted against extracellular microorganisms, whereas the immune response against intracellular microorganisms is mainly driven by cellular immune mechanisms. In addition, the distinction between the Th1 and Th2 cellular immune responses is complex for some of the antigens or immunogens included in vaccines that induce cellular as well as humoral immune responses, and it is not yet clear the degree of independence between antibody-mediated and cell-mediated immune responses under physiological conditions [48, 49].
Considering the variety of broad interactions of B lymphocytes with cellular immunity, B cells could have a significant impact on the outcome of airborne challenge with M. tuberculosis as well as the resultant inflammatory response . Therefore, we expect for peptides of Rv0679c to induce an immune response where humoral and cellular immunity are not mutually excluded.
The identification of Rv0679c HABPs capable of inhibiting target cell invasion by M. tuberculosis via host-cell receptor interactions supports their inclusion in further immunological studies in animal models aimed at evaluating their potential as components of a subunit-based antituberculous vaccine.
The wholehearted assistance of Nora Martinez in the translation and revision of this manuscript is greatly appreciated.
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