Mutation aa.no EcFtsZ | aa no. MjFtsZ | Location on FtsZ | Comple ment? 42° (2) | GTPase %WT | Asmb. GTP Mg | Asmb. GTP Ca | Asmb. GTP, 0.06 Dd | Asmb. GTP, 0.6 Dd | Asmb. GDP, 0.6 Dd | Refs |
|---|
Wild type | | | + | 100 | PF (3) | + | S (3) | T (3) | T (3) | (4) |
|---|
Benign mutations |
|---|
A70T(Z1) | A97 | Top-G | + (c) | 14 (a); <10 (d) | PF | | S | T | T | a/c/d |
A81V/F268C | A108 | Top | + | 15 (d) | | | | | | |
(Z100) | | (Buried) | | | | | | | | |
D158A | D185 | Front | + | 120 | PF | | S(-) | T(-) | T(-) | a |
D158A | | | + | 155 (b) | | | | | | b |
D158N | | | + | | | | | | | b |
D187A | D212 | Back-G | + | | PF | | S | T | T | a |
F268C(Z114) | E293 | BtmRtBk | + (c) | 70 (d) | | | | | | c/d |
D269A | D294 | BtmRtBk | + | 10 | PF | | S | T | T | a |
D299A | D324 | Back | +/? | 200 | PF | | S | T | T | a |
GTP contact mutations
|
N43D | N70 | Buried-Gγ | - | 31 (b) | | | | | | b |
D45A | D72 | Top-Gγ | - | 5 | NONE | + | T | T | T | a |
D45N | | | - | 5 (b) | | | | | | b |
G105S(Z84) | G132 | Top-G | +TS(f) | ∼ 10 (g) | | | S | S | T | f/g/a |
T108A(Z3) | T135 | Buried-G | - | ∼ 0 (c) | | | | NONE | | c/e |
N165D/Y | E192 | Buried-G | - | 17 (b) | | | | | | b |
N207D | N233 | Btm-Gsyn | - | 5 (b) | | | | | | b |
D209A | D235 | Btm-Gsyn | - | 7 | PF | + | T | T | T | a |
D209N | | | - | | | | | | | b |
D212G(Z2) | D238 | Btm-Gsyn | +TS2(c) | 0.5 (h) | | | | T | | c/e/h |
D212A | | | - | 7 | NONE | + | S+T | T | T | a |
D212N | | | 35 | S (i) | | | | | | i |
D212C | | | 17 | S (i) | | | | | | i |
D212E | | | 17 | | | | | | | i |
Lateral mutations
|
D86K | E113 | Left | - | 49 | twPF+T | + | S+T | S+T | T | a |
D96A | D123 | Left | - | | PF | + | S | NONE | | a |
D166K/F268V | E193/E293 | Right | - | 15 | PF | + | S (-) | S (-) | NONE | a |
E238A | E264 | Right | - | 145 | PF | + | S | T | T | a |
S245F | N271 | Right | - | 75 | PF+T | + | S | T | T | a |
E250A | D276 | Right | - | 67 | PF | + | S (-) | S (-) | T (-) | a |
E250K/D253K | D276/D278 | Right | - | 23 | PF | + | S (-) | S (-) | NONE | a |
- 1. Mutated amino acids are all surface residues,and their locations on the atomic structure are shown in Fig. 1. Top = the top surface, forming theinterface in the protofilament; most "Top" amino acids tested alsocontact the GTP, as indicated by Top-G; N43 and D45 probably contactthe gamma phosphate, indicated by -Gγ. Btm = the bottom surface,the other interface in the protofilament. N207, D209 and D212 formthe "synergy" loop and probably contact the GTP of the subunit below;these are indicated Btm-Gsyn. Front = the front surface (correspondingto the outside of the microtubule). Back = the back surface (insideof the microtubule). Right = the right lateral surface. Left = theleft lateral surface. N165 is largely buried, and makes contactwith the GDP (buried-G). 2. Complementation tests in the presentstudy (ref. a) were done with ftsZ84 (Ts) mutant cells.The mutant FtsZ was on the pBS58 plasmid. + indicates that the mutantplasmid supported cell growth and division in liquid culture overnightat 42°C. - indicates that the mutant gave only filamentous cellswith limited growth. Complementations in ref. b were done with both ftsZ84 anda genomic FtsZ null, with identical results. A blank indicates thatthis was not tested; TS = temperature sensitive. 3. Assembly wasin MEMK 6.5, with 1 mg/ml FtsZ and 2 mM GTP or GDP, and monitored byelectron microscopy. A blank in any assembly condition means thiswas not tested, and NONE means no polymers were found by electronmicroscopy. Assembly in GTP (without Ca or DEAE dextran) producedsingle protofilaments (PF) in wild type and most mutants. D86K producedtwined protofilaments (twPF). Assembly in 20 mM Ca produced protofilamentbundles when indicated by a +. Assembly in DEAE dextran normallyproduced sheets of protofilaments (S) at 0.06 mg/ml, and tubes (T,protofilaments in the curved conformation) at 0.6 mg/ml. 4. References:a, the present work; b, Table 2 of Wang et al., [];c, Bi and Lutkenhaus, [,];d, Fig.5B of Dai et al.[]; e, Mukherjeeet al, []; f, Phoenix and Drapeau [] and Powell and Court [];g, RayChaudhuri and Park, []; and de Boeret al., []; h, Trusca et al., []; i, Scheffers et al., [].
