Figure 4
Analysis of native parasitic PP5. The following protein fractions from Pf were analyzed by SDS-PAGE followed by staining (lanes 1–4) or Western blot using a non-immune antibody (lane 9) or anti-PP5 antibody (lanes 5–8; 10–13): (1) S100 extract of Pf cells; (2) partially purified native PfPP5 protein; (3) corresponding protein fraction from uninfected RBC; (4) purified recombinant (His)6PfPP5; (5–8) same fractions as lanes 1–4, respectively; (9) same fraction as in lane 1; (10–13) about 30 μg total protein from asynchronous, late trophozoite, late schizont / segmented, and ring stage Pf, respectively. The Mr of protein markers are shown on left in units of thousands.