Figure 3From: Acquisition of tolerance against oxidative damage in Saccharomyces cerevisiaeInduction of Hsp104 and Hsp26 expression in SOD and TPS1 strains. Total cellular proteins from strains EG103 (control strain), EG110 (sod2), EG118 (sod1), EG133 (sod1/sod2), JT2700 (control strain) and JT9060 (tps1) were analyzed by Western blot with anti-Hsp104 and anti-Hsp26 protein antibody. Lane1- extracts from cultures at exponential growth on glucose; Lane2- extracts from cultures at exponential growth on glucose directly submitted to 5 mM H2O2; Lane3- extracts from cultures exposed to a mild heat treatment at 40°C/1 h and Lane4- extract from cultures pre-exposed to 40°/1 h and then submitted to a lethal treatment with 5 mM H2O2. Each lane contain the same quantity of protein (50 μg of protein).Back to article page